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Lab Key Terms

TermDefinition
CODIS The FBI Laboratory's Combined DNA Index System (CODIS) blends forensic Science and computer technology into an effective tool for solving crime.
RFLP RFLPs are restriction fragment length polymorphisms, which are variations in the short base sequences where restriction enzymes can cut DNA.
Sticky Ends properties of the end of a molecule of DNA or a recombinant DNA molecule
DNA Fingerprinting technique employed by forensic scientists to assist in the identification of individuals by their respective DNA profiles
Cleave division between two things
DNA molecule that encodes the genetic instructions used in the development and functioning of all known living organisms
Comb makes wells in gel for gel electrophoresis
Restriction Enzyme locate specific sequences of DNA on which to bind and subsequently cleave
Exclusion no two processes or threads (henceforth referred to only as processes) are in their critical section at the same time
Hybridization two closely related species get together and mate, creating a hybrid
VNTR location in a genome where a short nucleotide sequence is organized as a tandem repeat
Nucleotides organic molecules that serve as the monomers, or subunits, of nucleic acids like DNA and RNA
Gel Lanes lanes DNA run in gel electrophoresis
DNA Fragments piece of a DNA strand
Palindrome nucleic acid sequence
Wells depressions in gel to place DNA in when doing gel electrophoresis
STR molecular biology method used to compare specific loci on DNA from two or more samples
Electric Field field generated by electrically charged particles and time-varying magnetic fields
Inclusion complex in which one chemical compound (the "host") forms a cavity in which molecules of a second "guest" compound are located
Microlieter 1/1000 of a mililiter
Human Genome complete set of genetic information for humans
Blunt Cut cut of the simplest part of the DNA strand
Buffer aqueous solution consisting of a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid
Recombinant DNA DNA molecules formed by laboratory methods of genetic recombination
DNA Polymerase cellular or viral polymerase enzyme that synthesizes DNA molecules from their nucleotide building blocks
Gene molecular unit of heredity of a living organism
Digital Micropipet Digital Pipette that only takes up units of microliters.
Staggered Cut cuts that give a single stranded "tail"
Agarose Gel polysaccharide polymer material, generally extracted from seaweed
inconclusive unable to derive a conclusion
fingerprint impression left by the friction ridges of a human finger
luminol used by forensic investigators to detect trace amounts of blood left at crime scenes, as it reacts with iron found in hemoglobin
PCR biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence
Restriction Site site where the DNA is cut
Gel Electrophoresis method for separation and analysis of macromolecules ( DNA, RNA and proteins) and their fragments, based on their size and charge
Evidence support or counter a scientific theory or hypothesis
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