Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Microbiology
Chapter 4 Micro
| Question | Answer |
|---|---|
| Refraction | Bending/changing the angle of light as it passes through medium(e.g lens) |
| Why is oil used on the oil immersion lens? | Prevent refractive loss and increases numerical apparatuses. |
| Bright Field microscopy | Images formed when light is transmitted through specimen |
| Phase-contrast Microscopy | Changes in light wavelength passing through a specimen are transformed into differences in light intensity(brightness) |
| Dark field Microscopy | "stop" disk added to condenser to block all but peripheral light from entering objective lens |
| Reverse image | Specimen appears bright on a dark background |
| Nomarski Microscopy | Provides 3-d image **uses 2 prisms and 2 beams of light |
| Fluorescence Microscopy | uses UV radiation and dyes |
| Confocal Scanning Laser Microscopy | *Provides detailed 3d, sectional images of an internal thicker structure *uses 2 dyes (green to view bacteria and Red to view dying bacteria) |
| Electron Microscopy | uses electromagnetic lenses, electrons, and fluorescence screen *Resolution is ~0.3nm & magnification 5k-1 million for biological speciment |
| Transmission Electron Microscopy | Used for fine detail inside cell, electrons pass through specimen |
| Scanning Electron Microscopy | Views detail on surface of specimen *specimen coated with metal (eg gold) |
| what are stains made of? | Organic salts |
| Basic dyes | bind to cell structure that carry (-) charge *commonly stain the cell |
| Acidic dyes | Repelled by cell structures that carry (-) charge *commonly stain the background around the cell |
| What are the three bacteria used in 1st lab? | 1)Serratia marcescens 2)Micrococcus luteus 3)Bacillus megaterium |
| Serratia marcescens | Red. Rod shaped. Gram - Causes UTI |
| Micrococcus luteus | Yellow. Wet colony. Pure culture. Round shaped. Found in air and dust. Rare to get an infection from it, but if you do it's skin infection. Gram + |
| Bacillus megaterium | Milky White. Rod shaped. Big. Forms spores. Found in Soil. Doesn't cause disease. Gram + |
| Negative Staining | Dye settles around specimen. Useful to detect capsule(glycocalyx) |
| Differential Staining | Primary dye and counter stain (eg Gram Stain) |
| Gram Stain | Permits differentiation of major categories of bacteria *Gram + and gram - |
| Gram Positive | Thick peptidal glycogen. Cell stain purple |
| Gram Negative | Thin peptidal glycogen. Cell stain pink |
| Ziehl-Neelsen acid-fast stain | identifications of diagnosis of clinical specimens |
| Mycolic Acid | Thick and waxy cell wall. (eg TB bacteria) |
| Schaeffer-Fulton endospore stain | Dye is forced by hear into resistant bodies called spores. *spores stain green *Vegetative cells stain pink |
| Flagella Stain | Staining increases diameter of flagella and visibility *Electromicroscope is ideal to view flagella |
| how many micro meters in 1 M? | 1*10^6 |
| how many nanometers in 1 M? | 1*10^9 |
| What can be seen under TEM? | atoms, amino acids, DNA, proteins, ribosomes, virus,mitochondria, chloroplast, RBC |
| What can be seen under LM? | Bacteria, archaea, chloroplasts, RBC, mitochondrion, large protozoa, flea |
| Purpose of Blue Filter that is Placed over light source? | to limit longer wavelengths from entering specimen |
| Whats the primary dye in Ziehl-Neelsen acid fast stain? | Carbol Fuchsin, colors bacteria red |
| Whats the decolorizer in Ziehl-Neelsen acid fast stain? | Acid alcohol, removes stain from non acid-fast bacteria |
| Whats the counter stain in Ziehl-Neelsen acid fast stain? | Methylene blue, colors non acid-fast bacteria blue and colors acid-fast bacteria red |
| Resolving power/Resolution | Differentiate between 2 objects close together |
| Red light | longer wavelength |
| Blue light | shorter wavelength(better resolution) |
| What's the purpose of heating the slide? | Fixes cell to slide so it won't wash off |
Created by:
izis
Popular Biology sets