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Microscope

Bio

TermDefinition
Microscope One of biology's most important tools. Refers to an instrument made up of a series of lenses held together in a tube. Useful for learning biological concepts and techniques. Magnifications range from 2X to over 100,000X
Magnifying Glass The simplest kind of magnifying lens
Compound Microscope The first microscope used in lab. Magnifications vary from about 40X to 450X
Electron Microscope Can magnify over 100,000X
Monocular Compound Microscope Objects examined must be s small or thin that light can pass through them to your eye. See and distinguish form and structure bc some parts absorb more light than others (transmitted light)
"Monocular" Image is viewed with one eye
"Compound" Refers to the image being produced by the interaction of several lenses
Magnification The degree to which an objects image is enlarged under the microscope
Resolution The ability of a microscope to reveal fine detail which permits us to clearly separate small details present in the image of the object
Working Distance The distance between the objective and the cover slip or the specimen when lenses are in correct focus. The distance varies with each objective magnification
Parfocal A magnification system where little adjustment in focus must be made when changing from one objective to another
Field of View The circle of light seen when looking through the microscope
Wet Mount A glass microscope slide with a specimen, a drop of water, and a cover slip
Arm A curved structure that supports the lenses, state, and focus knobs. It provides a connection between the lenses and a handle to grasp the microscope
Base The bottom part of the microscope that supports the entire instrument
Coarse Focus Knob The large knob that moves the stage up or down in order to focus. It should never be used when the high power objective is in position
Condenser The small cylindrical structure with chrome adjustment handle that is directly beneath the stage. Has a lens that focuses the light from the lamp.
Diaphragm Regulates and controls the amount of light passing through the specimen (entering the slide through the hole in the stage). Found directly below the condenser. Controls the amount of light passing up from the lamp to the stage.
Fine Focus Knob The smaller knob that moves the stage through very slight distances when focusing. Sharpens the image and provides exact focus
Lamp The light source that is located on top of the base. Turned on and off by the power switch. There is a red wheel on the right side of the base that adjusts the intensity of the light.
Objectives Contains lenses of different magnifications. Attached to revolving nosepiece.
Scanning Objective 4X lens (shortest). Observe and scan large areas
Low Power (L.P.) Objective 10X
High Power (H.P.) Objective 40X
Ocular (eyepiece) Tops lens and uppermost part of the microscope. This lens magnifies 10X and it is also used for viewing the specimen. It has a pointer inside
Power Switch A small toggle button at the back of the base that turns the microscopes lamp on/off
Revolving Nosepiece Holds the objectives and allows them to be easily interchanged. Each objective clicks into place when ready for viewing.
Stage A square, black platform located below the objective lenses. Supports the slide and contains an opening that admits light from the lamp
Stage Adjustment Knobs 2 knobs located underneath the stage on the left side of the microscope. Upper knob moves the stage forward and backward this moving your specimens image up/down. Lower knob moves the stage and image from side to side
Stage Clip A silver arm on the top of the stage that holds a slide firmly in place against a silver plate
Created by: sotti988
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