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Ch. 15: Genes & How
BSC 1010
| Question | Answer |
|---|---|
| Which part of the prokaryote RNA polymerase holoenzyme is involved with recognizing promoters specific to certain types of genes? | Sigma |
| In prokaryotes, RNA polymerase can bind "naked" sequences upstream of promoters. Eukaryotes need protein "decorations" to facilitate RNA polymerase binding. What is the name of these proteins which assist RNA polymerase with binding? | Transcription factors |
| human genome was first sequenced, scientists expected to find around 100,000 genes. Upon its completion and analysis of reading frames, they discovered the number was more likely 25,000. | Alternative splicing |
| Which is responsible for the production of a peptide bond between adjacent amino acids during translation? | Peptidyl transferase activity of the large subunit. |
| T/F: The central dogma of molecular biology states that DNA is transcribed to make mRNA, which is translated to make a protein. | True |
| Which of the following pieces of evidence is among the strongest that supports the idea that all organisms have arisen from a single distant ancestor? | The code is universal. Genes can be transferred from one organism to another and can be successfully expressed in their new host |
| The region of a gene which "tells" RNA polymerase where the genetic message is located is called the | promoter. |
| Regarding gene expression, which of these accurately distinguish prokaryotes from eukaryotes? | Prokaryotic ribosomes translate mRNA transcripts that are still being synthesized by RNA polymerase. |
| How might a molecular biologist most easily purify mRNA from all the other kinds of RNA in a eukaryotic cell? | She could create a filter covered with long poly-T sequences and run a cell extract over it. The tails of mRNA would base-pair with the filter and be retained, while all the other types of RNA would be rinsed off. |
| If pre-mRNA is hybridized with mature mRNA, regions loop out and can be viewed with an electron microscope. What do the looped out regions represent? | Introns to be excised later. |
| T/F: Connection of an amino acid to a tRNA is a spontaneous reaction. | False |
| "Wobble" pairing is largely responsible for | the redundancy of the genetic code. |
| A nonsense mutation typically involves | inappropriate termination of translation early in the "reading frame". |
| The area on a tRNA molecule which connects to a specific mRNA molecule is called | DNA |
| Which of the following is a nucleic acid found in ribosomes and is an important building block of ribosomes? | rRNA |
| Name the ribosomal binding sites | P site, A site, E site. |
| Which of the following statements about eukaryotic mRNA is TRUE? a cap is added to their 5' end a poly-A tail is added to their 3' end each usually specifies only a single protein A and B | a cap is added to their 5' end, a poly-A tail is added to their 3' end ,each usually specifies only a single protein |
| Splicing joins together | two exons. |
| Which of these events occur as a prokaryotic mRNA is being transcribed? splicing addition of a cap addition of a poly-A tail binding of ribosomes more than one of the above | binding of ribosomes |
| T/F: Processing of eukaryotic mRNA occurs in the cytoplasm prior to translation. | False |
| T/F: Operons are found in eukaryotes but not in prokaryotes. | False |
| The nucleic acid sequence in mRNA is determined by | nucleotide sequence in DNA; mRNA is made complementary to the nucleotide sequence in DNA. |
| If a frameshift mutation causes a stop codon to be inserted into the DNA sequence | the resulting protein will be too short and non-functional; The resulting protein will stop being made before it is complete. It will be too short and nonfunctional. |
| A nucleotide deletion in DNA replication causes | the amino acids inserted after the deletion to be incorrect; The amino acids coded for before the deletion will not be affected and will still be correct. |
| T/F: The addition of a single nucleotide to the DNA sequence causes a frameshift mutation. | True; A frameshift mutation occurs when one nucleotide is added and all of the following groupings of codons are changed. All of the amino acids inserted after the addition will be incorrect. |
| T/F:In a frameshift mutation all of the amino acids before the shift are changed. | False; The amino acids coded for before the deletion will not be affected and will still be correct. |