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MOLB Chapter 3

Observing Microorganisms Through a Microscope

Simple microscope has only one lens
Compound Microscope has 2 or more lenses
Compound Light Microscope used to observe living organisms, resolution up to .2 micrometers, magnifies up to 2000x, requires stains
Darkfield microscope able to see things you cannot see in CLM because of the dark background
Phase contrast microscope similar to CLM, shows 3D image and internal structures
Fluorescence Microscopy uses higher wavelengths of light than can be absorbed
Confocal stain specimen, hit with blue light and reconstruct with a computer to get 3D view
Transmission Electron Microscopy magnify 10,000x resolution 20nm, look inside cells
Scanning Electron Microscopy 10 nm resolution, magnification 10,000, allows view of cell surface
Staining coloring organisms with the application of dye
Smear applying organisms to a slide
Fixed act of attaching the specimen to the slide
need for staining live/unstained cells have little contrast with surrounding medium, making them difficult to see, researchers use staining to make discoveries about cell behavior by observing specimens
Basic dyes chromaphore is a cation (+)
acidic dye chromaphore is an anion (-)
Negative stain staining only the background
simple stains only use one dye
Mordant amplifies appearance of the primary stain
Created by: 100000860991270