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MicrobiologyLab11

Questions about "Smear Preparation and Simple Staining"

QuestionAnswer
Bacterial cells are difficult to see because their index of refraction is very similar to ________. water or air
A good spear should appear only slightly ________. turbid
A slant culture is generally considered a better source of organisms for staining than is a ________ culture. broth
An important consideration for the slide used for staining is that it be ________. clean
True or false: Tap water can be used to make smears because there are too few bacteria (about 10,000 per ml) to see under oil immersion. True.
A smear is best made if during the heating stage (mild/high) heat is used. mild
True or false: Virtually everyone has bacteria in their saliva. True.
The number of cells cited in the introduction (500,000 per ml) will result in about 1 cell per oil immersion field when observed under the microscope. If you had ten cells per field, you would have about ________ bacteria per ml of your saliva. 5,000,000
The large nucleated cells observed in saliva are most likely ________. squamous
If cells stained with methylene blue are washed too long, the dye will be ________. removed or washed away
Created by: NeuroHeart
 

 



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