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Mircobilogy Lab

TermDefinition
Catalase Test microorganisms produce hydrogen peroxide. Accumulation of this can result in death unless it degrades the hydrogen peroxide.
Superoxide Dismutase Aerobic organisms that lack catalase can degrade toxic superoxides.
Catalase Production If catalse present, the chemical reaction will develop bubbles of oxygen gas. This is a positive reaction. If no bubbles it is negative. Can do test in tryptic soy agar slant.
Staphlococcus composed of both pathogenic and nonpathogenic organisms. 3 major groups S. aureus, S. saprophyticus, S. epidermis.
S. Epidermidis skin lesions and endocarditis
S. saprophyticus urinary tract infections
S. aureus infection abscesses, boils, carbuncles, acne
pneumonia infection of internal organs and tissues
osteomyelitis abscesses in bone and bone marrow
endocarditis inflammation of the endocardium
cystitis inflammation of the urinary bladder
pyelonephritis inflammation of the kidneys
staph enteritis contaminated foods, septicemia
S. aureus /coagulase has coagulase- forms clot
S. aureus color ,growth, and fermentation golden yellow/ growth positive, + fermentation
S. aureus hemolysis beta
S. aureus novobiocin sensitivity sensitive
S. epidermidis color, growth, fermentation white, +, -
S. epidermidis novobiocin sensitivity sensitive
S. saprophyticus color white, +, -
S. saprophyticus novobiocin sensitivity resistant
Coagulase + Staph produce hydrolytic enzyme Dnase
Coagulase test used to ? determine S. aureus strain and so does DNase test
Novobiocin sensitivity used to distinguish S. epidermidis from S. saprophyticus
Streptococci description circular, translucent to opaque chains. All members of gram positive and require nutrient rich media (blood agar)
Streptococci classified by 2 means Hemolytic activity and serologic activity
Strep. pyogenes human repiratory infection tonsilitis, bronchopneumonia, scarlet fever, and skin disorder cellulitis
Strep uses Bacitracin sensitivity test test for beta-hemolysis (destruction of red blood cell/clear zone)
Strep uses Directigen test test for Group A Antigen.
Camp test for Group B streptococci produce arrowhead zone of hemolysis
Strep Group D Bile esculin test checks for bile producing brown/black color. Lack of color means non Group D
6.5 sodium chloride test GOLD growth + = Group D Strep no growth= non group D
Strep. pneumoniae major hemolytic pathogen in humans, usually inflammation of bronchial and alveolar membranes
S. pneumoniae forms large thick capsules and produces alpha hemolysis on blood agar
Pneumonococcus Infection lung infections, otitis media, meningitis.
Created by: Mel87
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