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Microbiology 1

QuestionAnswer
nosocomial infections are hospital derived
virulence factors and pathogenicity asks the question...and the answer is usually... what causes microbes to cause dieseases?...usually specific proteins make microbes pathogenic
microbial antagonism is what gives certain microbes the advantage to grow and also includes how to prevent pathogens
enzymes are...that... proteins...catalyze reactions
aseptic technique involves how to prevent nosocomial infections
scientific method helps prove pathogenicity
antoni van leeuwenhoek began making and using...and he hoften made a... simple microscopes...new microscope for each specimen
leeuwenhoek examined...and visualized...that he called... water...tiny animals, fungi, algae, and single-celled protozoa...animalcules
by the end of the...these organisms were called... 19th century...microorganisms
carolus linnaeus developed...which was used for... taxonomic system...naming plants and animals and grouping similar organisms together
through linnaeus' taxonomic system, leeuwenhoek's microorganisms got goruped into...which include... 6 categories...fungi, protozoa, algae, bacteria, archaea and small multicellular animals
protozoa are eukaryotic and single celled
algae are...with... microbes...chlorophyl for making own energy
bacteria are single celled and prokaryotic
archaea are similar to bacteria
small multicellular animals include parastitic worms
the golden age of microbiology focused on what 4 questions is spontaneous generation of microbial life possible? what causes fermentation? what causes disease? how can we prevent infenction and disease?
some philosophers and scientists of the past thought living things arose from three processes that include asexual repro, sexual repro, or from nonliving matter
aristotle proposed...which means that... spontaneous generation...living things can arise from nonliving matter
francesco redi's experiment tested...and involved... meat to disprove aristotle's theory...leaving meat uncovered, air-tight sealing it and exposing it to air through gauze
pasteur's experiment involved...that demonstrated... swan-necked flasks...that no microbial growth appeared
in pasteur's experiment, when the flask was tilted... dust from the bend in the neck seeped back into the flask and made the infusion cloudy with microbes w/i a day
the scientific method was partially developed due to the debate over spontaneous generation
scientific method step 1 observation to question
scientific method step 2 question (broad) to hypothesis (specific)
scientific method step 3 hypothesis to experiment, incluidng control groups
scientific method step 4 experiment , incluidng control groups to observations to experimental data support hypothesis? then to accepting hypothesis, then repeat experiment -> accepting hypothesis then it becomes a theory of law
if the experimental data does not support the hypothesis then you ... reject the hypothesis and modify it until it gives a positive outcome
pasteur applied the scientific method while trying to figure out what causes fermentation
pasteur's fermentation experiment involved...and ended up giving off 4 facts... boiling the grape juice to sufficiently kill microbes...spontaneous fermentation does not happen, air does not ferment grape juice, bacteria ferment grape juice into acids, yeast ferments grape juice into alcohol
ignaz semmelweiss was concerned about the...so he postulated that some... high mortality rate of mothers in maternity wards...cadaverous material carried by physicians from the autopsy room caused childbed fever
semmelweiss advocated the washing of hands in bleach between performing autopsies and working w/ patients
mortality rates dropped...because of semmelweiss and handwashing 90%
pasteur also developed... germ theory of disease
robert koch studied...such as... causative agents of disease...anthrax and colonies of microorganisms
koch's postulates: suspected causative agent must be.... found in every case of the disease and be absent from healthy hosts
koch's postulates: agent must be... isolated and grown outside the host
koch's postulates: when agent is introduced into a healthy, susceptible host... the host must get the disease
koch's postulates: the same agent must be... found in the diseased experimental host
how can we prevent infection and disease? semmelweiss and...lister's...nightingale and...snow...jenner's... handwashing...antiseptic technique...nursing (cleanliness)...infection and epidemiology...vaccine (field of immunology)
bacteriology bacteria and archaea
phycology algae
mycology fungi
protozoacology protozoa
parasitology parasitic protozoa and parasitic animals
virology viruses
microbial metabolism biochemistry, chemical reactions within cells
microbial genetics functions of DNA and RNA
environmental microbiology relationships between microbes and aong microbes, other organisms and their environment
serology helps us to..and it is the study of... prevent against disease...blood serum and its antibodies
which two scientists discovered the existence in the blood of chemicals and cells that fight infection? von behring and kitasato
immunology helps to...and is the study of... prevent against disease...the body's defense against specific pathogens
chemotherapy helps to...and involves... help against disease...chemicals
in chemotherapy, fleming discovered...and domagk discovered... penicillin...sulfa drugs
bacteria are mostly...,their size is about...and they cause things like... free living...2 microns...plague, tuberculosis and anthrax
fungi are...and..., their size is...and they cause things like... free living and eukaryotic...3-5 microns...candidiasis and histoplasmosis
protozoa are mostly...and range from...and cause... free living...1-300 microns...malaria, sleeping sickness and toxoplasmosis
viruses are strictly...which means they need intracellular...a host to replicate
virus size ranges from...and causes things like... 20-200 nm...ebola, AIDS, avian influenza
light microscope has a max resolution of...and there is a constraint due to... 1000 fold...wavelength of the light
light microscope stains adhere to...defining... specific cellular structure...structure and composition
can color be seen in light microscopes? yes
transmission electron microscopes have the...which is up to.. greatest magnification...1 million x mag
tem electrons have high energy and short wavelength
can tems see color? what do they see? no...density
scanning electron microscopes involve different... sample prep and configuration of detector
sem electrons bounce off the surface of specimen
only...are visible with the sem surface features
environmental microbiology martinus beijerinck and sergei winogradsky
biochemistry louis pasteur and eduard buchner
chemotherapy paul ehrlich
immunology edward jenner
public health microbiology john snow
etiology robert koch
epidemiology john snow
biotechnology louis pasteur
food microbiology louis pasteur
Created by: handrzej
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