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Biology OCR AS
| Question | Answer |
|---|---|
| Define: Magnification | - Degree to which size of an image is larger than the object size |
| Define: Resolution | - Degree to which it is possible to distinguish between two objects that are very close together - the higher the resolution, the greater the detail that you can see |
| Define: Staining | Coloured stains - chemicals that bind to chemicals on or in specimen allowing specimen to be seen. Some stains bind the cell structures |
| Define: Sectioning | - Specimens embedded in wax - thin sections are cut without distortion of the structure of the specimen |
| Formula for Image Size | Image size = Actual Size x Magnification |
| Why do we need to stain molecules | Most biogical molecules are not coloured and are transparent so we need to add stains to make them visible under a microscope |
| Three rules of cell theory developed by Schwann | - All living things consist of cells - New cells form from the division of pre-existing cells - Cells contain information that acts as the instruction for growth - this information can be passed onto new cells |
| What is a graticule and describe it | A graticule is tiny transparent thing with a ruler etched on it which can be used to work out structure sizes. Stage Micrometer required to calibrate it (EPU) |
| Describe Transmission Electron Microscope | - Electron beam passes through a very thin prepared sample - Electrons pass through denser parts less easily giving contrast - Thus image is 2 D |
| Describe a Scanning Electron Microscope | - Electron beam directed onto a sample - electrons don't pass through - They bounce off - Final image is 3D |
| Advantages of Electron Microscopes | - Resolution is 0.1nm - Can produce detailed images of organelle structure - SEM produces 3D images which can reveal details of contours and cellular tissue arrangements |
| Limitations of Electron Microscopes | - Electron deflected by molecules of air so sample needs to be in vacuum - Expensive - Preparing samples and using it is very expensive requiring a high degree of skill and training |
| What is the resolution for a light microscope | 200nm |
| What is the magnification for a light microscope | 1500X |
| What is the resolution of an electron microscope | 0.2nm |
| What is the magnification of a Transmission Electron Microscope | x500,000 |
| What is the magnification of a Scanning Electron Microscope | x100,000 |
| What is the function of the plasma membrane | Regulates movement of substances into and out of the cell - Have receptor molecules on them which allow them to respond to chemicals like hormones |
| Describe structure of the plasma membrane | -membrane found on surface of namimal cells and inside cell wall of plant cells and prokayotic cells - Made mainly of lipids and proteins |
| What is the function of the cell wall | Supports the plant cell and keeps the cells turgid |
| Describe structure of the cell wall | Rigid structure made mainly of the carbohydrate cellulose |
| Describe the function of the nucleus | - Chromatin made from proteins and DNA (DNA controls cell activity) - Pores allow substances like RNA to move between the nucleus and the cytoplasm - Nucleolus makes ribosomes |
| Describe the structure of the nucleus | - Large organelle - Contains a nuclear envelope - Contains many nuclear pores - Contains chromatin - Often a structure called a nucleolus |
| Describe the function of a lysosome | Contains digestive enzymes kept separate from cytoplasm - Can be used to digest invading cells or to break down worn out components of the cell |
| Describe the structure of a lysosome | - Round organelle - Surrounded by a membrane with no clear internal structure |
| Describe the function of a ribosome | The site where proteins are made or synthesised |
| Describe the structure of a ribosome | Very small organelle that either floats free in cytoplasm or is attached to the rough endoplasmic reticulum |
| Describe the function of the Rough Endoplasmic Reticulum | Folds and processes proteins that have been made at the ribosomes |
| Describe the structure of the rough endoplasmic reticulum | - System of membranes enclosing a fluid filled space - Surface covered in ribsomes |
| Describe the function of the smooth endoplasmic reticulum | Synthesizes and processes lipids |
| Describe the structure of the smooth endoplasmic reticulum | - System of membranes enclosing a fluid filled space - Surface has no ribosomes |
| Describe the function of a vesicle | Transports substances in and out of the cell via the plasma membrane and between organelles - Some formed by Golgi apparatus or the endoplasmic reticulum, while others formed at cell surface |
| Describe the structure of a vesicle | A small fluid filled sac in the cytoplasm surrounded by a membrane |
| Describe the function of the golgi apparatus | It processes and packages new lipids and proteins and also makes lysosomes |
| Describe the function of the mitochondria | Site of aerobic respiration - where ATP is produces - Large numbers found in active cells like muscle or sperm |
| Describe the structure of a mitrochondria | - Usually oval shaped - Have a double membrane with the inner one folded to form cristae - Inside is the matrix which contains enzymes involved in respiration |
| Describe the function of the chloroplasts | - Site where photosynthesis takes place - Some parts take place in the grana while the other parts take place in the stroma |
| Describe the structure of the chloroplasts | - Small, flattened structure in plant cells - Surrounded by a double membrane - Also has membranes inside called thylakoid membranes which are stacked up in some parts of the chloroplast to form grana - Grana linked together by Lamellae |
| Describe the function of the centriole | Involved with the separation of chromosomes during cell division |
| Describe the structure of the centriole | Small, hollow cylinders, containing a ring of microtubules |
| Describe the function of the cilia | - The microtubules allow the cilia to move - Movement is used by the cell to move substances along the cell surface |
| Describe the structure of the cilia | - Small hair like structures found on the surface membrane - Outer membrane and a ring of 9 pairs of microtublues with a pair in the centre - Only in animal cells |
| Describe the function of flagellum | Microtubules contract to make the flagellum move - used like a motor to help the prokaryote swim |
| Describe the function of undulipodium | Microtubules contract to make the flagellum move - helps sperm to swim |
| Describe the structure of undulipodium | Similar structure to cilia, just a bit longer |