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Micro Lab 24 & 25
Chemical Methods of Control: Disinfectants and Antiseptics & Antimicrobial Drugs
| Question | Answer |
|---|---|
| chemicals available for controlling the growth of microbes | antimicrobial agents |
| chemical agents used on inanimate objects to lower the level of microbes on their surfaces | disinfectants |
| chemicals used on living tissue to decrease the number of microbes | antiseptics |
| chemicals that result in bacterial death | bactericidal agents |
| chemicals causing temporary inhibition of growth of bacteria | bacteriostatic agents |
| standard method for measuring the effectiveness of a chemical agent, uses Salmonella enterica, Staphylococcus aureus, and Pseudomonas aeruginosa and is limited to bactericidal compounds and cannot be used to evaluate bacteriostatic compounds. | American Official Analytical Chemist's use-dilution test |
| measures what concentration level is effective after 10 mins at 20*C | Use-dilution test method |
| against life | antibiosis |
| inhibiting substance produced by a microorganism that inhibits other microorganisms | antibiotics |
| antimicrobial chemicals used internally whether natural (antibiotics) or synthetic | antimicrobial drugs |
| disease-causing organism | pathogen |
| zone inhibition affected by diffusion rates and growth rate of organism and minimum inhibitory concentration. | disk-diffusion method |
| antimicrobial agent diffuses from high concentration to low concentration - effective agent will inhibit growth & measurements can be made of the size around the disks | zones of inhibition |
| standardized test for agar diffusion methods | Kirby-Bauer test |
| allows the antimicrobial agent to diffuse freely in the Kirby-Bauer test | Mueller-Hinton agar |
| determined by testing for bacterial growth in dilutions of the antibiotic in nutrient broth | minimum inhibitory concentration (MIC) |
| chemical drugs | chemotherapeutic agent |