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Micro Lab 14

Fermentation

QuestionAnswer
used to detect acid and gas production from carbohydrates Fermentation tube
used to distinguish organisms that produce large amounts of acid from glucose and organisms that produce the neutral product acetoin MRVP tests
pH above 6 turns yellow, red color indicates pH below 4.4 Methyl red test
contains peptone, an acid-base indicator (phenol red), an inverted tube to trap gas, and .5-1% of desired carbohydrate. Fermentation medium
fermentation that results in acid production will turn the indicator what color? pH? Yellow; pH of 6.8 or below
When gas is produced during fermentation some will be trapped in the what? inverted (Durham) tube
occurs with or without oxygen present Fermentation
Neutralization of the indicator in fermentation because of ammonia production turns it what color? Red
acetoin present (neutral pH) it will turn red - no acetoin (acidic pH) it will turn a light brown Voges-Proskauer test
Bacteria with what enzyme can break down citrate into pyruvate - which can then be reduced in fermentation? citrate lyase
When bacteria use citrate and ammonium, the medium is what because of what? alkalized; ammonia (NH3)
What is the indicator for a citrate test and what color does it change when it is alkalized - is it a negative or positive citrate utilization test? Bromthymol blue; blue; positive
Created by: hollieritter
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