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Micro Lab 15 & 16
Protein Catabolism, Part 1 & 2
| Question | Answer |
|---|---|
| large organic molecules (cellular enzymes) | Proteins |
| subunits that make up a protein and consist of carbon, hydrogen, oxygen, nitrogen, and, sometimes, sulfer and are used primarily in anabolic reactions. | amino acids |
| bond amino acids together | peptide bonds |
| amino acids bonded together forming a small chain | peptide |
| amino acids bonded together forming a larger molecule | polypeptide |
| dissolves in warm water (50*C), solidifies (gels) when cooled below 25*C and liquifies (sols) when heated to about 25*C | Nutrient gelatin |
| can be used by bacteria as carbon and energy sources when carbohydrates aren’t available. | amino acids |
| a waste production of protein digestion in most vertebrates and is excreted in the urine from the kidneys | Urea |
| liberates ammonia from urea and is a useful diagnostic test for identifying bacteria | urease |
| contains peptone, glucose, urea, and phenol red (which turns yellow because the pH of the prepared medium is 6.8) | Urea agar |
| During incubation, bacteria possessing what will produce what and do what to the pH of the medium turning the indicator what color at what pH? | urease; ammonia; raises the pH and turns the indicator fuchsia (hot pink) because the pH is at 8.4 |
| shows whether or not bacteria have the necessary enzymes to hydrolyze large protein molecules. | Gelatin hydrolysis |
| exuded by bacteria that will liquefy gelatin and doesn’t solidify even when cooled to lower temperatures. | hydrolytic exoenzyme |
| the removal of an amino group, converted into ammonia and can be excreted from cell - results in the formation of an organic acid. | deamination |
| deamination can also be ascertained by testing for the presence of what using what and turns what color? | ammonia; Nessler's reagent; deep yellow in the presence of ammonia |
| the removal of carbon dioxide from an amino acid | decarboxylation |
| remove hydrogen sulfide from the sulfur-containing amino acids: cysteine and methionine | desulfurase enzymes |
| commonly called rotten-egg gas because of the copious amounts liberated when eggs are decomposed | Hydrogen sulfide (H2S) |
| a heavy-metal salt containing ferrous ion (Fe2) is added to a nutrient culture medium to detect what production? It produces a visible what? | H2S (hydrogen sulfide); black precipitate |
| What test is performed by inoculation a bacterium into tryptone broth and detecting what by the addition of dimethylaminobenzaldehyde aka what? | indole test; detecting indole; Kovac's reagent |
| a differential screening medium that is a single culture medium in which motility, indole production, and ornithine decarboxylase activity can be determined | MIO |
| causes indole producing bacteria to turn RED after what has been added? | Kovac's reagent |
Created by:
hollieritter
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