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Lecture #8
DNA Structures
| Question | Answer |
|---|---|
| What is DNA? | A double helix made of antiparallel nucleotides polymers. |
| what are the four criteria genetic must meet? | Information, Replication, Transmission, and Variation. |
| What are Rough Strains? | Appears rough and do no secrete capsules and infections are not fatal. |
| What are Smooth Strains? | Appears smooth and secrete capsules and infections are fatal. |
| Where are DNA found? | Inside the capsid. |
| What are DNA build of? | Nucleotides |
| What is the genome? | It is a complete complement of an organism genetic materials. |
| What are the 3 components of nucleotides? | 1. Phosphate 2. Nitrogenous Base 3. Pentose Sugar |
| What is the sugar in DNA and RNA? | DNA- deoxyribose RNA- ribose |
| What are two base that makes up purines? and for purimidines? | DNA base - 1. Purines - Adenine and Guanine 2. Purimidines - Cytosine and Thymine |
| What is the base in RNA for pyrimidines? | Unlike DNA, RNA has base Uracil instead of Thymine. |
| Differences in DNA and RNA? | RNA has ribose sugar instead of deoxyribose; has Uracil base instead of Thymine and RNA is usually single stranded. |
| What carbon is base and phosphate attach to? | Base - 1 carbon Phosphate - 5 carbon |
| What are the components that form the backbone of strand structure? | phosphate and sugars form the backbone of DNA strand and has directional from 5 to 3. |
| What the ratios of the nucleotides occur in? | T+C = A+G A=T G=C purines always base pair with pyrimidines |
| How many H bonds does A and T has? and G and C? | A and T has 2 Hydrogen bonds G and C has 3 Hydrogen bonds |
| How many base pairs are there per turn? | 10 base pairs occur per turn |
| Example of two DNA complementary strands | 5-GCGGATTT-3 3-CGCCTAAA-5 these two strands are ANTIPARALLEL |
| What is the major groove in the DNA strand? | It is where protein binds and effect gene expression. |
| DNA Structure | It is a redundancy with a profound purpose strand DNA is susceptible to both chemical and physical damage. The complementary will used to fix the damage. It doesn't matter which strand is damage. |
| What is the purpose of Helix? | It prevents the DNA from becoming tied into knots. |
| Denaturation of DNA | breaking down DNA structure and the melting temperature is directly proportional to base composition. |
| Renaturation of DNA | kinetics of renaturation is related to concentration single vs multi-copy genes *the higher in concentration of the DNA, the faster single stranded molecules reanneal. |
| What are the three levels of DNA composition? | 1. DNA wrapping - DNA wrapped around histones to form nucleosome 2. 30-nm fiber - shorten length another 7 fold 3. Radial Loop Domains - interaction between 30nm fibers and nuclear matrix |
| What happens when cell gets ready to divide? | Chromosomes are more compacted, euchromatin is lightly packed, and heterochromatin is much more compacted. |