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Micro-Lab

Microbes in the environment

QuestionAnswer
What quantity of agar will you need to add? 1.28 g
What effect does the agar have on the culture medium? It solidifies the medium
To what temperature will it have to be heated? 100 degrees celcius
Why is it necessary to keep the flask at an angle through this procedure? To minimize contamination from the air
Why is the swab first moistened in sterile water before sampling a dry surface? the swab must be moist to adequately pick up microbes.
What is human body temperature in degrees celcius? 37 degrees celcius
Why is condensation on the agar undesirable? Bacteria will be in a liquid suspension instead of forming discrete colonies and it will spread the microbes on the surface
What is the use of Petri plates in microbiology? They provide a large surface area for solid culture media in an easily stored container
What are bacteria using for nutrients in nutrient agar? Peptone and beef extract
What is the purpose of the agar? The agar is the solidifying agent.
Why is agar preferable to gelatin as a solidifying agent in culture media? 1. It liquefies at 100 degrees Celsius and stays in a liquid state until cooled to 40 degrees Celsius. 2. Once hardened, it stays solid up to 100 degrees Celsius. 3. Few microbes can metabolize the agar, so it remains solid.
Did all the organisms living in or on the environments sampled grow on your nutrient agar? Why or why not? No because we didnt satisfy all the nutritional and environmental requirements
How could you tell whether the turbidity in your nutrient broth was from a mixture of different microbes or from the growth of only one kind of microbe? By sub-culturing the nutrient broth onto the surface of a nutrient agar plate. The plate will create discrete colonies & if they differ u can tell.
Created by: 100001455498513
 

 



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