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Bio Lab Exam
Practical Exam
| Question | Answer |
|---|---|
| Parts of the scientific method | 1. Making observations, 2. Formulating a Hypothesis, 3. Experimental Design, 4. Data Collection, 5. Data Analysis, 6. Make Conclusions, 7. Repeat |
| Four C's that embody a good scientist | Curious, collecting information, communicating the results, comfortable with new information |
| Three basic factors that are absolutely necessary for designing experiments | Observations, Hypothesis, experimental design |
| Correct pipette for volume | 2 mL blue, 5 mL green |
| Lambert-Beer Law | States that under three specific conditions the absorbance of a solution is directly and linearly related to its concentration |
| Under what conditions is the Lambert-Beer Law applicable | 1. Monochromatic light is used (one wavelength at a time) 2. The absorbance is measured at the wavelength of maximum absorbance for the solution 3. The absorbance is less than 1.0 |
| DCIP | 2,6-dichloro-indophenol |
| What is DCIP used for | Measure the photosynthetic rate |
| What is photosynthesis | Light energy + water + CO2 --> Glucose + Oxygen Light energy + 6H20 + 6C02 --> C6H12O6 + 6O2 |
| Standard curve | Graph of absorbance vs. concentration |
| Field of view | The amount of the substance that is viewable depending on the magnification |
| Resolving power | Ability to distinguish detail |
| Course control | Used for initial focusing |
| Fine Control | Used for fine tuning focus |
| Working distance | Distance between objective lens and specimen |
| Parfocalism | Ability of a lens to remain basically in focus when switching from one objective lens to another |
| Field diameter | Distance across the field of view |
| Microscope safety rules | Always carry the microscope with two hands (one under the base and one under the arm), Only clean lens with lens paper, Clean lens in a circular motion, Never use course control on high power, Be careful with glass slides, Keep both eyes open, Begin low |
| PARTS OF MICROSCOPE PAGE 20 | |
| Compound light microscope | Two or more lenses uses light rays and glass lenses to magnify small objects or sections of specimens |
| Stereoscopic microscope | Dissecting microscope is used to observe objects that are too large or thick for the compound light microscope |
| Total magnification | Multiply ocular number by objective |
| Osmosis | Passive transport |
| What are enzymes made of | They are proteins made of amino acids |
| What is the function of a catalyst | Speeds up a reaction |
| Turnip extract (peroxidase) | Catalyst |
| H2O2 | Substrate |
| Guaiacol | Dye |
| Aerobic respiration | Glucose + Oxygen --> Carbon dioxide + ATP + Heat |
| End products of fermentation | Ethanol and CO2 or Lactic acid (animals) |
| Three major steps in aerobic respiration | Glycolysis, krebs cycle, ETC |
| Glycolysis | Conversion of glucose to pyruvate |
| Krebs cycle | Oxidizing pyruvate to CO2 |
| ETC | Generates protein gradients to form ATP |
| Primary electron carrier in respiration | NADH |
Created by:
AliRutherford
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