Busy. Please wait.

show password
Forgot Password?

Don't have an account?  Sign up 

Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.

By signing up, I agree to StudyStack's Terms of Service and Privacy Policy.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.

Remove ads
Don't know
remaining cards
To flip the current card, click it or press the Spacebar key.  To move the current card to one of the three colored boxes, click on the box.  You may also press the UP ARROW key to move the card to the "Know" box, the DOWN ARROW key to move the card to the "Don't know" box, or the RIGHT ARROW key to move the card to the Remaining box.  You may also click on the card displayed in any of the three boxes to bring that card back to the center.

Pass complete!

"Know" box contains:
Time elapsed:
restart all cards

Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

DNA 3 Mitochondrial

Lecture 4.

Comparison of Human DNA 3.2 billion bp, Mito=16568 2 per cell and Mito-500to1000 DNA-99.75%
What are Mitochondrial Functions? Power house of cell. Provides all chemical energy in form of ATP. Other functions include Apoptosis, heat production, storage of calcium, elongation of fatty acids, steroid synthesis.
What are the characteristics of mtDNA? Has:Cirular Genome, about 16569 BP, codes for 37 genes, 22 tRNAs, 2 RNAs, 13 proteins. 4-5 copies per Mitochondria, thousands per cell. mtDNA makes up less than 1% of DNA. Some people have a 9 BP deletion.
Mitochondria has two strands describe them. The Heavy Strand (H) which contains greater amounts of guanine and is purine rich. 28 gene products encoded. The Light Strand (L) which contains more C and T nucleotides. Is pyrimidine rich. 9 (8tRNAs and 1 protein from L)
What is the reference sequence? Reference sequence is also known as the Anderson sequence Sequenced in 1981. Also called Cambridge reference sequence. The revised reference sequence is accepted standard (is 16568 BP)
Compare CRS to rCRA. No difference in the control region. Original CRS contained a CC at 3106-3197 (only 1 C) (1 BP shorter)
How is mtDNA inherited? Only through the female line from your mother.
How do we measure mtDNA variance? Restriction Fragment Length Polymophism: Used in old method of measuring mtDNA variation. New method consists of using PCR and sequence analysis in control region. MT control region only varies 1-2% in people.
What are some problems or what can go wrong with mtDNA? Polymeric C Stretches, Hetroplasmy, Sample Mixtures, Nuclear pseudogenes, Common types of mtDNA.
Go over Nuculear Pseudogenes, C stretch, Same
Future of mtDNA use? Whole mitochondria genome sequencing. Proposed by Deborah Nickerson group in 1998. Used 24 pairs to span mt Genome. Method used by Ingman in 2000 sequence using 53 mt Genomes.
What are the mtDNA databases we have? EMPOP=European DNA Profiling group Mitochondrial DNA Populace Databases Project.
Created by: 1649820075