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Quiz 11-7-07
Microbiology
| Questions | Answers |
|---|---|
| Name 2 ways of destroying microorganisms and Viruses using heat? | ~ Moist heat, and Dry Heat |
| In moist heat, ? and ? destroys microorganisms by coagulating their proteins. | ~ boiling water, and steam |
| Will boiling water kill endospores? | ~ No |
| Name 3 examples of moist heat methods? | ~ boiling, pasteurization, and pressurized steam (autoclaves) |
| Dry heat is the absence of ?, burns cell parts or irreversibly denatures ? ? | ~ moisture, proteins |
| What is an example of dry heat? | ~ flaming your wire loop |
| What is a chemical that destroys all microorganisms, INCLUDING ENDOSPORES? | ~ Sterilants |
| What kills microorganisms and viruses, but doesn't reliably kill endospores? | ~ High Level Disinfectant |
| What kills microorganisms, but doesn't reliably kill all viruses? | ~ Intermediate Level Disinfectant |
| Low Level Disinfectants can kill: ? | ~ fungi, most vegetative bacteria, and enveloped viruses. |
| Low Level Disinfectants can not kill what type of vegetative bacteria? | ~ mycobacteria |
| What does Low Level Disinfectants NOT kill? | ~ Endospores, and reliably kill nakes viruses |
| Name 5 things to consider when selecting the appropriate germicidal chemical? | ~ Toxicity, Activity (in the presence of organic matter), Compatibility with treated material, Residue, and Cost & Availability |
| Name two filters we can use for filtration on fluids? | ~ Depth Filters, and Membrane Filters |
| In Depth Filters, complex passages retain ? and allow ? to pass. | ~ microorganisms, fluid |
| What are two things that depth filters can't do? | ~ reliably remove viruses, and remove bacteria from enzymes |
| Membrane Filters have tiny pores/holes that are used to trap ? and ? | ~ bacteria, and viruses |
| By filtering fluids, ? may be unnecessary. | ~ pastuerization |
| What is a type of air filter we use to remove nearly all microorganisms that has a diameter greater than ? ? | ~ HEPA Filters, .3 microns |
| HEPA stands for:? | ~ High Effiency Particulate Air Filters |
| What is a type radiation that causes biological damage by creating free radicals, used to sterilize heat sensitive materials, and decreases microorganisms in food? | ~ Gamma Irradition |
| Ultraviolet Irradition dames the structure and function of ?? | ~ nucleic acids |
| What would we use to get rid of microorganisms only in the air and on the surface of an object. | ~ Ultraviolet Irradition |
| What doesn't kill microorganism directly (rather by the heat produced in the product)? | ~ Microwaves |
| What 3 chemical preservatives prevent microbial growth? | ~ Benzoic acid, Sorbic acid, and propionic acid |
| What 2 chemical preservatives inhibit the germination of endospores and the growth of Clostidium Botulinum? | ~ Nitrate, Nirtite |
| Nitrate and Nitrate give meat a ? coloration. | ~ pink |
| To preserve foods, we can add ? or ? which does what? | ~ sugar, or salt; draws H2O out of cells reducing availabilty for microbes. |
| To preserve food we can dry food by ? and ? | ~ dessication, or lyophilizaiton |