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Quiz 11-7-07

Microbiology

QuestionsAnswers
Name 2 ways of destroying microorganisms and Viruses using heat? ~ Moist heat, and Dry Heat
In moist heat, ? and ? destroys microorganisms by coagulating their proteins. ~ boiling water, and steam
Will boiling water kill endospores? ~ No
Name 3 examples of moist heat methods? ~ boiling, pasteurization, and pressurized steam (autoclaves)
Dry heat is the absence of ?, burns cell parts or irreversibly denatures ? ? ~ moisture, proteins
What is an example of dry heat? ~ flaming your wire loop
What is a chemical that destroys all microorganisms, INCLUDING ENDOSPORES? ~ Sterilants
What kills microorganisms and viruses, but doesn't reliably kill endospores? ~ High Level Disinfectant
What kills microorganisms, but doesn't reliably kill all viruses? ~ Intermediate Level Disinfectant
Low Level Disinfectants can kill: ? ~ fungi, most vegetative bacteria, and enveloped viruses.
Low Level Disinfectants can not kill what type of vegetative bacteria? ~ mycobacteria
What does Low Level Disinfectants NOT kill? ~ Endospores, and reliably kill nakes viruses
Name 5 things to consider when selecting the appropriate germicidal chemical? ~ Toxicity, Activity (in the presence of organic matter), Compatibility with treated material, Residue, and Cost & Availability
Name two filters we can use for filtration on fluids? ~ Depth Filters, and Membrane Filters
In Depth Filters, complex passages retain ? and allow ? to pass. ~ microorganisms, fluid
What are two things that depth filters can't do? ~ reliably remove viruses, and remove bacteria from enzymes
Membrane Filters have tiny pores/holes that are used to trap ? and ? ~ bacteria, and viruses
By filtering fluids, ? may be unnecessary. ~ pastuerization
What is a type of air filter we use to remove nearly all microorganisms that has a diameter greater than ? ? ~ HEPA Filters, .3 microns
HEPA stands for:? ~ High Effiency Particulate Air Filters
What is a type radiation that causes biological damage by creating free radicals, used to sterilize heat sensitive materials, and decreases microorganisms in food? ~ Gamma Irradition
Ultraviolet Irradition dames the structure and function of ?? ~ nucleic acids
What would we use to get rid of microorganisms only in the air and on the surface of an object. ~ Ultraviolet Irradition
What doesn't kill microorganism directly (rather by the heat produced in the product)? ~ Microwaves
What 3 chemical preservatives prevent microbial growth? ~ Benzoic acid, Sorbic acid, and propionic acid
What 2 chemical preservatives inhibit the germination of endospores and the growth of Clostidium Botulinum? ~ Nitrate, Nirtite
Nitrate and Nitrate give meat a ? coloration. ~ pink
To preserve foods, we can add ? or ? which does what? ~ sugar, or salt; draws H2O out of cells reducing availabilty for microbes.
To preserve food we can dry food by ? and ? ~ dessication, or lyophilizaiton
Created by: Patrick24g
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