microbiology testing Word Scramble
|
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Question | Answer |
what is the products formed from sugar fermentation test? | Acid with or without gas |
what is a positive result of sugar fermentation test? | red indicator turns yellow |
what is the purpose of sugar fermentation test? | production of acid and/or gas in fermintation |
what are possible mediums for sugar fermentation? | phenol red glucose broth, phenol red lactose broth or phenol red mannitol broth |
what is a negative result of sugar fermentation test? | red indicator remains red |
what is a negative result of Methyl Red test? | Broth remains yellowish/orange or has no color |
what is a negative result of Voges-Proskauer test? | Broth turns a dark brown/green color |
what is a negative result of Catalase test? | no bubbles visible |
what is a negative result of Oxidase test? | no color change within 30 sec |
what is a negative result of Starch Hydrolysis test? | no clear zone (entire plate is dark blue) |
what is a negative result of Gelatin Hydrolysis test? | no liquefaction of medium |
what is a negative result of Indole Production test (Tryptophen Hydrolysis)? | Yellow layer on the top of the culture |
what is a negative result of Urease test? | no color change to medium (yellow-orange) |
what is a negative result of H2S test? | no black precipitate (possible red/yellow change) |
what is a negative result of Citrate Utilization test? | Medium is unchanged, lite green color |
what is a negative result of Litmus Milk Reaction Coagulation test (Curd)? | Medium is unchanged |
what is a negative result of Litmus Milk Reaction Peptonization test (Casein Hydrolysis)? | Medium is unchanged |
what is a negative result of Litmus Milk Reaction Litmus Reduction test? | Medium is unchanged |
what is a negative result of Litmus Milk Reaction pH change test? | Medium is unchanged |
what is a positive result of Litmus Milk Reaction pH change test? | acid = PINK alkaline= PURPLE |
what is a positive result of Litmus Milk Reaction Litmus Reduction test? | litmus changed to WHITE (changed by oxygen being used up) |
what is a positive result of Litmus Milk Reaction Peptonization test (Casein Hydrolysis)? | becomes Transparent (due to hydrolysis of casein) |
what is a positive result of Litmus Milk Reaction Coagulation test (Curd)? | Curd formation, whay liquid maybe presence on surface |
what is a positive result of Citrate Utilization test? | medium will turn Prussian BLUE |
what is a positive result of H2S test? | Black precipitate formed (iron sulfide) |
what is a positive result of Urease test? | medium turns HOT PINK |
what is a positive result of Indole Production test (Tryptophen Hydrolysis)? | red layer on the top of the culture |
what is a positive result of Gelatin Hydrolysis test? | liquefaction of medium |
what is a positive result of starch hydrolysis test? | a clear zone next to bacterial growth |
what is a positive result of oxidase test ? | organism turns PURPLE |
what is a positive result of catalase test ? | bubbles visible (immediate) |
what is a positive result of Voges-Proskauer test? | Broth turns RED |
what is a positive result of Methyl Red Test ? | Broth turns PINK/RED |
2 tests can be performed on MRVP Broth, what are those tests? | 1. Methyl Red (test for LOTS of ACID) 2. Voges-Proskauer (acetoin production, 2-3 Butanediol Fermentation) |
The Methyl Red and Voges-Proskauer tests both use what same Medium? | MRVP Broth |
Methyl Red Test:Name of MEDIUM? | MR-VP Broth |
Methyl Red Test:Important INGREDIENTS in Medium? | 1 carbohydrate (glucose, lactose, or sucrose), peptone, phenol red indicator and Durham tube (only in glucose tube) |
Methyl Red Test:How to INOCULATE? | Loop inoculate broth |
Methyl Red Test:How to INCUBATE Medium for Test? | incubate at 35°C for 48 hours |
How do you perform the Methyl Red test? | 1. After incubation, transfer 1 mL of broth into a NEW sterile TUBE 2. Add 4-5 drops of Methyl Red 3. Read color results |
How do you interpret the Methyl Red test RESULTS? | Methyl Red colors: (+) RED pH<= 4.4 (-) YELLOW pH = 6.2 (-) NO COLOR CHANGE (no mixed acid fermentation) |
T/F ==CHEMICAL==If bacteria CAN do MIXED ACID FERMENTATION, acid BYPRODUCTS will OVERCOME PHOSPHATE BUFFER (LOWERS pH), ELSE pH REMAIN SAME | True |
T/F ==METABOLIC==Fermentation is a metabolic process in which ORGANIC MOLECULES act as ELECTRON DONOR (OXIDIZED) and 1 or more ORGANIC PRODUCTS act as FINAL ELECTRON ACCEPTOR. | True |
T/F Usually if (+) for Methyl Red test, then (-) for Voges-Proskauer cuz look for different acids | True |
1 pH CHANGE is A LOT. By what FACTOR is this? Also how many more times acidic is a change from 6 pH to 4 pH? | 1 pH change = 10 times 6 to 4 pH is a 100 times more acidic |
How to read results of Indole? | After adding Kovac's: red layer on top -> positive for Indole -> Trytophanase hydrolyzes tryptophan Yellow layer on top-> Indole negative -> Tryptophanase absent. |
Blackening of the medium?? | H2S is produced. |
How to read methyl red test? | After adding methyl red: : MR positive= turn red (pH 4.0) MR negative= turn to any other color (pH 6.0 or higher) |
How to read Voges Proskauer test? | After adding Reagent A and B: : VP postitive=Turn red ->Acetoin VP negative=If no red color developed. -> no acetoin |
VP test what? | Acetoin. |
VP reagents? | VP A: Alpha Naphthol. VP B: Potassium hydroxide. |
Purpose of Citrate test? | Tosee if organism use citrate as its own carbon source. |
How to read result of Citrate Utilization? | Negative for Citrate Utilization: No growth, green color of medium. Positive: Growth on the slant, medium could be green color or blue color. |
Why use Indole? | used to identify if organism is capable of breaking down tryptophan to pyruvic acid, indole, ammonia by using enzyme tryptophanase. |
Why use Methol red? | used to see if bacteria produce acid from glucose |
Why use Voges Proskauer? | used to identify if org produce acetoin from 2,3-butanediol fermentation. |
Why use Citrate? | to see if org use citrate as it own carbon source. |
What products are formed from the Methyl Red test? | Mixture of acids |
What is the medium for Methyl red test? | MR-VP Broth |
What is the medium for Voges-Proskauer test? | MR-VP Broth |
What is the reagent of Methyl Red test? | MR red indicator |
What is the reagent for Voges-Proskauer test? | Barretts A and Barretts B |
What is the reagent for Catalase test? | Hydrogen Peroxide |
What is the reagent for Oxidase test? | Oxidase reagent |
What is the reagent for starch hydrolysis test? | Grams iodine |
What is the reagent for indole production test? | Kovac's reagent |
what product is formed from Voges-Proskauer test? | aceton |
what is the product from the Methyl Red test? | mixture of acids |
what is the product from the catalase test? | oxygen |
what is the product from the oxidase test? | none |
Created by:
jmabert
Popular Biology sets