ASCP MLT BOC Microbiology

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Question

Answer

What is Steam Under Pressure sterilization?

Autoclave,15 pounds of pressure for 15 minutes, 121°C. Kills spores

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4 components of Gram stain

Crystal violet, Iodine, 95% ethyl alcohol, and Safranin

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S. aureus identification

yellow and beta hemolytic colonies, catalase and coagulase positive, ferments mannitol

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S. epidermidis

White colonies, nonhemolytic, Non pathogenic. Cat pos, coag neg, manitol neg sensitive to novobiocin

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S. saprophyticus

White to slightly yellow colonies, nonhemolytic, caog neg, may ferment mannitol, resistant to novobiocin

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Micrococcus

Gram pos cocci in pairs, tetrads, or clusters, Pigmented (yellow, orange, tan), nonpathogenic, cat pos, coag neg, oxidative, sensitive to bacitracin and lysozyme.

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Group A strep

pinpoint colonies, grayish white, translucent, beta hemolytic, PYR pos, Bacitracin susceptible S. pyogenes

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Group B strep

larger than Strep A,bulls eye colonies, diffuse beta hemolysis, Na hippurate and CAMP pos, S. agalactiae

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Group D strep

Slightly opaque, nonhemolyic

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Strep. pneumoniae

Gram pos lancet shaped cocci in pairs, colonies are round, translucent, dome shaped when young, central depression with age. Encapsulated, Bile solubility pos, optichin sensitive

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Strep. viridans

alpha hemolytic, normal flora, resistant to optichin and bile solubility neg

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Enterococcus

nonhemolytic, hydrolyzes esculin, grows in NaCl broth, PYR positive

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Bacillus anthracis

Gram pos rods with square ends,spore forming, anthrax, Aerobic, medusa head colonies, nonhemolytic

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Corynebacteria spp

Nonspore forming GPR, chinese letter or palisade formation, C. diphtheriae, black colonies with brown halos on Tindale's agar, black/grey coloneis on cystinetellurite, cat pos, nonmotile

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Erisipelothrix

GPR pleomorphic, nonspore forming, cat neg, H2S pos, nonmotile, Indole neg, resistant to neomycin, Erysipeloid=skin infection from handling fish or animals

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Lactobacillus

GPR pleomorphic, vaginal flora, cat neg, chains of rods in THIO, pinpoint alpha-hep colonies to rough grey colonies

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Listeria monocytogenes

GPcoccobacilli, pleomorphic, nonsporeforming, meningitis, BAP and CNA, tiny colonies with narrow zone of betahemolysis, esculin pos, cat pos, motile

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Nocardia

GPos, fine branching filaments with fragmentation, slow growing, Grows well on Sabouraud dextrose agar, BCYE, Marin-Lewis, CNA, acid fast, sulfur granules

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N. gonorhoeae

Gneg diplococci, grows on choc and selective media, Superoxol-pos, Sugar utilization, glucose pos

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N. meningitis

G neg diplococci, choc and selective media, may grow on BAP with increases CO2, sugar utilization, glucose and maltose pos

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N. lactamica

Gneg diplococcigrows on selective media, sugar utilization, glucose pos, maltose and lactose slow pos, ONPG pos

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Moraxella catarrhaliz

Gneg diplococci, grows on BAP, choc, and gonococcal selective media, Hockey puck colonies, cat and oxidase pos, neg for all sugars and ONPG. DNase-pos

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Kingella denitrificans

GNR grows on gonococcal selective media, oxidase pos, cat neg, delayed glucose pos

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