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ASCP Microbiology

ASCP MLT BOC Microbiology

QuestionAnswer
What is Steam Under Pressure sterilization? Autoclave,15 pounds of pressure for 15 minutes, 121°C. Kills spores
4 components of Gram stain Crystal violet, Iodine, 95% ethyl alcohol, and Safranin
S. aureus identification yellow and beta hemolytic colonies, catalase and coagulase positive, ferments mannitol
S. epidermidis White colonies, nonhemolytic, Non pathogenic. Cat pos, coag neg, manitol neg sensitive to novobiocin
S. saprophyticus White to slightly yellow colonies, nonhemolytic, caog neg, may ferment mannitol, resistant to novobiocin
Micrococcus Gram pos cocci in pairs, tetrads, or clusters, Pigmented (yellow, orange, tan), nonpathogenic, cat pos, coag neg, oxidative, sensitive to bacitracin and lysozyme.
Group A strep pinpoint colonies, grayish white, translucent, beta hemolytic, PYR pos, Bacitracin susceptible S. pyogenes
Group B strep larger than Strep A,bulls eye colonies, diffuse beta hemolysis, Na hippurate and CAMP pos, S. agalactiae
Group D strep Slightly opaque, nonhemolyic
Strep. pneumoniae Gram pos lancet shaped cocci in pairs, colonies are round, translucent, dome shaped when young, central depression with age. Encapsulated, Bile solubility pos, optichin sensitive
Strep. viridans alpha hemolytic, normal flora, resistant to optichin and bile solubility neg
Enterococcus nonhemolytic, hydrolyzes esculin, grows in NaCl broth, PYR positive
Bacillus anthracis Gram pos rods with square ends,spore forming, anthrax, Aerobic, medusa head colonies, nonhemolytic
Corynebacteria spp Nonspore forming GPR, chinese letter or palisade formation, C. diphtheriae, black colonies with brown halos on Tindale's agar, black/grey coloneis on cystinetellurite, cat pos, nonmotile
Erisipelothrix GPR pleomorphic, nonspore forming, cat neg, H2S pos, nonmotile, Indole neg, resistant to neomycin, Erysipeloid=skin infection from handling fish or animals
Lactobacillus GPR pleomorphic, vaginal flora, cat neg, chains of rods in THIO, pinpoint alpha-hep colonies to rough grey colonies
Listeria monocytogenes GPcoccobacilli, pleomorphic, nonsporeforming, meningitis, BAP and CNA, tiny colonies with narrow zone of betahemolysis, esculin pos, cat pos, motile
Nocardia GPos, fine branching filaments with fragmentation, slow growing, Grows well on Sabouraud dextrose agar, BCYE, Marin-Lewis, CNA, acid fast, sulfur granules
N. gonorhoeae Gneg diplococci, grows on choc and selective media, Superoxol-pos, Sugar utilization, glucose pos
N. meningitis G neg diplococci, choc and selective media, may grow on BAP with increases CO2, sugar utilization, glucose and maltose pos
N. lactamica Gneg diplococcigrows on selective media, sugar utilization, glucose pos, maltose and lactose slow pos, ONPG pos
Moraxella catarrhaliz Gneg diplococci, grows on BAP, choc, and gonococcal selective media, Hockey puck colonies, cat and oxidase pos, neg for all sugars and ONPG. DNase-pos
Kingella denitrificans GNR grows on gonococcal selective media, oxidase pos, cat neg, delayed glucose pos
Created by: nmcutler1
 

 



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