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What is Steam Under Pressure sterilization?
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4 components of Gram stain
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ASCP Microbiology
ASCP MLT BOC Microbiology
| Question | Answer |
|---|---|
| What is Steam Under Pressure sterilization? | Autoclave,15 pounds of pressure for 15 minutes, 121°C. Kills spores |
| 4 components of Gram stain | Crystal violet, Iodine, 95% ethyl alcohol, and Safranin |
| S. aureus identification | yellow and beta hemolytic colonies, catalase and coagulase positive, ferments mannitol |
| S. epidermidis | White colonies, nonhemolytic, Non pathogenic. Cat pos, coag neg, manitol neg sensitive to novobiocin |
| S. saprophyticus | White to slightly yellow colonies, nonhemolytic, caog neg, may ferment mannitol, resistant to novobiocin |
| Micrococcus | Gram pos cocci in pairs, tetrads, or clusters, Pigmented (yellow, orange, tan), nonpathogenic, cat pos, coag neg, oxidative, sensitive to bacitracin and lysozyme. |
| Group A strep | pinpoint colonies, grayish white, translucent, beta hemolytic, PYR pos, Bacitracin susceptible S. pyogenes |
| Group B strep | larger than Strep A,bulls eye colonies, diffuse beta hemolysis, Na hippurate and CAMP pos, S. agalactiae |
| Group D strep | Slightly opaque, nonhemolyic |
| Strep. pneumoniae | Gram pos lancet shaped cocci in pairs, colonies are round, translucent, dome shaped when young, central depression with age. Encapsulated, Bile solubility pos, optichin sensitive |
| Strep. viridans | alpha hemolytic, normal flora, resistant to optichin and bile solubility neg |
| Enterococcus | nonhemolytic, hydrolyzes esculin, grows in NaCl broth, PYR positive |
| Bacillus anthracis | Gram pos rods with square ends,spore forming, anthrax, Aerobic, medusa head colonies, nonhemolytic |
| Corynebacteria spp | Nonspore forming GPR, chinese letter or palisade formation, C. diphtheriae, black colonies with brown halos on Tindale's agar, black/grey coloneis on cystinetellurite, cat pos, nonmotile |
| Erisipelothrix | GPR pleomorphic, nonspore forming, cat neg, H2S pos, nonmotile, Indole neg, resistant to neomycin, Erysipeloid=skin infection from handling fish or animals |
| Lactobacillus | GPR pleomorphic, vaginal flora, cat neg, chains of rods in THIO, pinpoint alpha-hep colonies to rough grey colonies |
| Listeria monocytogenes | GPcoccobacilli, pleomorphic, nonsporeforming, meningitis, BAP and CNA, tiny colonies with narrow zone of betahemolysis, esculin pos, cat pos, motile |
| Nocardia | GPos, fine branching filaments with fragmentation, slow growing, Grows well on Sabouraud dextrose agar, BCYE, Marin-Lewis, CNA, acid fast, sulfur granules |
| N. gonorhoeae | Gneg diplococci, grows on choc and selective media, Superoxol-pos, Sugar utilization, glucose pos |
| N. meningitis | G neg diplococci, choc and selective media, may grow on BAP with increases CO2, sugar utilization, glucose and maltose pos |
| N. lactamica | Gneg diplococcigrows on selective media, sugar utilization, glucose pos, maltose and lactose slow pos, ONPG pos |
| Moraxella catarrhaliz | Gneg diplococci, grows on BAP, choc, and gonococcal selective media, Hockey puck colonies, cat and oxidase pos, neg for all sugars and ONPG. DNase-pos |
| Kingella denitrificans | GNR grows on gonococcal selective media, oxidase pos, cat neg, delayed glucose pos |
Created by:
nmcutler1