Micro204 sugar fermantaion lab 21
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Amino acid fermentation leads to the production of X and X. | Alpha-lheto acids, ammonia
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| the specific enzymes causing fermentation of sugar belongs to the X enzyme type. | endo-
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| If phenol red truns a magenta color, it means X has been produced. | pH change
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| The small inverted tube found within a larger tube is called a X and is used to trap X. | Durham tube, gas
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| When phenol red changes to a yellow color, this indicates that X has been produced. | acid
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| The two pH indicators that are most often used in a microbiology lab are X and X. | phenol red, bromeresol purple
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| The process of fermentation involves two groups of enzymes known as X and X. | endoenzymes, transport proteins
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| List the 4 essential parts of ingredients of a fermentation tube. | chem defined carbohydrate, pH indicator, inverted Durham tube, nutrient broth
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| List four ferment-able carbohydrates. | Lactose, fructose, fructons, polyols
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| X is the term that refers to the anaerobic breakdown of carbohydrates. | Fermentation
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| What colors denote a neutral, acid, or alkaline reaction? Neutral, acid, alkaline. pH of the color change | red, yellow, pink, 6.8 and lower
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| If a carbohydrate broth does not change color after it has been inoculated and incubated, how can you tell whether the unchanged color is due to failure of the organism to grow or failure to ferment the carbohydrate? | Turbidity or gas production.
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| Medium Used: | phenol red-glucose broth, phenol red-lactose broth, phenol red-sucrose broth
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| If positive, will be for | fermentation of sugar
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