Bio #14
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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Biotechnology | show 🗑
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Recombinant DNA Technology | show 🗑
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show | The deliberate modification of an organism's genome
using biotechnology techniques
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show | A technique used to amplify small
segments of DNA
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DNA Cloning and Recombinant DNA : Recombinant DNA (rDNA) | show 🗑
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DNA Cloning and Recombinant DNA : Plasmids | show 🗑
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show | A DNA molecule used to deliver genetic material into cells.
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show | The process of introducing recombinant DNA into a
host cell
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show | Proteins that cut DNA at specific sequences. These are used to "cut" both the plasmid and the gene of interest so they can be combined. Ex: EcoRI: Cuts DNA at a specific recognition sequence, leaving "sticky ends" that are joined with other DNA fragments
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DNA Ligase | show 🗑
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Polymerase Chain Reaction (PCR) : PCR | show 🗑
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PCR Steps | show 🗑
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show | Heating the DNA to separate strands
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PCR Annealing | show 🗑
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PCR Extension | show 🗑
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show | A technique used to separate DNA, RNA, or protein fragments by size and charge through a gel matrix under an electric field - Ethidium bromide or other dyes are used to visualize DNA bands
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show | The process of inserting a gene into a vector, introducing it into a host organism, and producing the gene product (protein) in large quantities - Bacterial Cloning: E. coli or other bacteria can be used as hosts for gene cloning
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show | Organisms whose genome has been altered by the
insertion of foreign genes. This can involve the creation of Transgenic Plants and animals
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show | Plants modified to express desirable traits (e.g.,
resistance to pests or herbicides)
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Transgenic Animals | show 🗑
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Applications of Biotechnology : Genetic Engineering of Organisms : Gene Therapy | show 🗑
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Somatic Cell Gene Therapy | show 🗑
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Germ-line Gene Therapy | show 🗑
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Applications of Biotechnology : Genetic Engineering of Organisms : Pharmaceutical Production | show 🗑
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show | The use of living organisms to detoxify or remove pollutants
from the environment (e.g., oil spills)
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Applications of Biotechnology : Genetic Engineering of Organisms : Environmental Biotechnology : Waste Treatment | show 🗑
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Genomics | show 🗑
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Human Genome Project (HGP) | show 🗑
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show | Determining the precise order of nucleotides in a DNA
molecule
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show | Comparing the genomes of different species to identify similarities and differences
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Functional Genomics | show 🗑
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show | Using techniques like microarrays or RNA-Seq to study gene activity and how genes are expressed under different conditions
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show | Personalized Medicine, Pharmacogenomics, Genomic Medicine, Agricultural Genomics
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Biotechnology | show 🗑
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show | DNA that is formed by combining genetic material from
different sources
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show | A small circular DNA molecule used as a vector in cloning.
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show | A technique used to amplify specific DNA
sequences
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show | Enzymes that cut DNA at specific sequences
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show | The process of producing identical copies of a gene
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show | Organisms that have been genetically modified with foreign
DNA
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show | The study of the complete set of genes in an organism’s genome
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show | A technique used to treat or prevent disease by modifying the
genetic material of a patient’s cells
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Or sort by any of the columns using the down arrow next to any column heading.
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Created by:
kassa.cool