Lab Practical 1
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| show | Sterility - prevent water condensation from the lid dropping onto the plate
Contamination Prevention - Requires bacteria to go through agar to get to bottom of the plate
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| Three types of media | show 🗑
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| show | agar, nutrients, salt, and pH buffers and tags
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| What is the streak method? | show 🗑
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| Pellicle Growth Pattern in Broth | show 🗑
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| Sediment Growth Pattern in Broth | show 🗑
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| show | grows throughout entire broth aggressively; ocular density is high
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| Flocculent Growth Pattern in Broth | show 🗑
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| Filiform Growth Pattern on a Slant | show 🗑
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| show | Tree-like with thick center branch
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| show | Beaded down the middle
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| show | Spread out growth from the center
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| show | Branch off from thin center line
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| show | Spiny from thick center line
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| Dilution Factor | show 🗑
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| show | prior dilution factor x dilution of current container
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| Standard CFU Formula | show 🗑
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| Range of Colony Count | show 🗑
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| show | Plate Count Agar - provides total bacterial count
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| show | Eosin Methylene Blue - purple agar plate inhibits gram + bacterial growth that differentiates lactose fermenters from non-fermenters
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| CNA | show 🗑
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| show | Uses multiple dyes to help differentiate media
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| When are Darkfield Microscopy used? | show 🗑
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| show | Used for wet mounts mostly and to determine size/shape of colony
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| show | Specimen on a slide with no more than one dye to stain, i.e. crystal violet
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| show | Brightfield, Phase-Contrast, Darkfield
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| Resulting color of a gram + bacteria | show 🗑
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| show | red/pink because the cell wall is thin and does not hold the crystal violet dye after the decolorizer step where acetone-alcohol is used, because the lipopolysaccharide layer is dissolved releasing the dye. Example: E. Coli
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| Which dye is used to reinforce crystal violet | show 🗑
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| show | Some bacteria are gram variable, but most will lean gram +; decolorizing agent was used for too long; culture is too old
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| Bacteria Counting Methods | show 🗑
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| Spectrophotometer (How it measures) | show 🗑
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| Chromogenesis | show 🗑
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| Measurements of Colony Morphology (Types) | show 🗑
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| show | round, irregular, filamentous (branched from center into a circle), rhizoid (random branches), curled
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| Types of Colony Edges | show 🗑
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| Types of Colony Elevation | show 🗑
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| Punctiform | show 🗑
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| show | Degrades H2O2 within the cell into O2 and H20 (test will have bubbles if positive). Enzyme is usually seen in gram +, for example staphylococcus
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| Catalase Test | show 🗑
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| show | Differentiates between Pseudomonadaceae (+ result, blue/purple) and Enterobacteriaceae (- result). Helps identify species with O2 as the final electron receptor because the redox reagent (dimethyl or tetramethyl-p-phenylenediamine dihydrochloride)
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| Restriction with Oxidase Test | show 🗑
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| show | tests for salt resistance and mannitol sugar use. Phenol red determines sugar usage with acid as the by product. The acid lowers the pH causing a yellow color for + results. If the colony grows on the plate, then it is halophilic as well.
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| Halophillic | show 🗑
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| show | Plate with DNA and methyl green indicator in it to test for presence of deoxyribonuclease (an exoenzyme) that breaks down DNA. If a DNAse enzyme is present, a yellow zone will appear around the growth area indicating +
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| show | phenol red sugar broth (lactose, sucrose, glucose/dextrose, mannitol) or sugar discs on a plate
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| show | Sugar broth with a phenol red indicator that will turn yellow (+ result) in the presence of carbohydrate utilization with an acid byproduct. A durham tube is used to check for CO2 utilization
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| Hanging Drop Mount (Purpose) | show 🗑
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| show | no flagella/non-motile
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| show | movement caused by the bouncing of bacteria by the movement of the fluid they are suspended within
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| show | run is when the motile bacteria moves towards the nutrient source and tumble is when the bacteria moves/rolls away from the nutrient source/threat
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| Monotrichous | show 🗑
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| Peritrichous | show 🗑
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Created by:
drubin676
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