Gel Electrophoresis
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| Electrophoresis allows for what on the basis of what? | show 🗑
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| show | anion
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| an anion will migrate toward what? also what is it called | show 🗑
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| show | cation
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| a cation will migrate toward what? and what is this called? | show 🗑
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| the migration and separation of molecules are carried out using a what? also what are some examples of these? | show 🗑
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| show | seiving effect, in which small molecules navigate the matrix more quickly than larger ones
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| show | proteins and nucleic acids
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| for now, we will be separating DNA fragments using ______ as the matrix | show 🗑
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| show | size, shape, % agarose, voltage
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| show | smaller fragments of DNA will travel further than the larger ones
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| show | plasmids; isolated plasmids
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| show | larger; not resolved; smaller; not retarded
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| what are two significant equations regarding voltage? | show 🗑
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| show | the higher the voltage, the greater the power, which creates lots of HEAT
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| show | smiling bands (heat is more rapidly dispersed at EDGES OF GEL)
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| what is the measurement of dispersion? | show 🗑
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| During electrophoresis water is_________, generating ________at the anode and ______ ______ at the cathode. This results in regions of high and low pH. Therefore a _______ is needed. | show 🗑
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| the two most popular buffers for agarose gel electrophoresis are _____ and _______. | show 🗑
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| show | glycerol and the dye bromophenol blue~200-400 bp)
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| show | intercalates; nucleic acids; absorption spectrum; fluoresce
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| show | band intensity
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| show | high molecular weight DNA
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| show | a smear
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| the molecules separate by ______ RELATIVE TO THE CONDITIONS in which the electrophoresis was conducted | show 🗑
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| A ____ _________ (a sample of DNA fragments of known sizes and mass) is used as a ________ to estimate the size of unknown DNA molecules | show 🗑
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