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Destiny Bus Terms

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Term
Definition
Codis   “Combined DNA Index System” is the generic term used to describe the FBI’s program of support for criminal justice DNA databases as well as the software used to run these databases.  
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RFLP   A variation in the length of restriction fragments produced by a given restriction enzyme in a sample of DNA. Such variation is used in forensic investigations and to map hereditary disease.  
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Sticky Ends   An end of a DNA double helix at which a few unpaired nucleotides of one strand extend beyond the other.  
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DNA Fingerprinting   The analysis of DNA from samples of body tissues or fluids in order to identify individuals.  
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Cleave   To split or sever (something), esp. along a natural line or grain.  
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DNA   Deoxyribonucleic acid, a self-replicating material present in nearly all living organisms as the main constituent of chromosomes. It is the carrier of genetic information.  
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Comb   To untangle, smooth, arrange, or fasten.  
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Restriction Enzyme   An enzyme produced chiefly by certain bacteria, having the property of cleaving DNA molecules at or near a specific sequence of bases.  
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Exclusion   The process or state of excluding or being excluded.  
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Hybridization   The act of mixing different species or varieties of animals or plants and thus to produce hybrids  
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VNTR   A Variable Number Tandem Repeat is a location in a genome where a short nucleotide sequence is organized as a tandem repeat. These can be found on many chromosomes, and often show variations in length between individuals  
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Nucleotides   A compound consisting of a nucleoside linked to a phosphate group. Nucleotides form the basic structural unit of nucleic acids such as DNA.  
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Gel Lanes    
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DNA Fragments   Tiny pieces of DNA  
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Palindrome   A word, phrase, or sequence that reads the same backward as forward, e.g., madam or nurses run.  
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Wells   (of a liquid) Rise to the surface and spill or be about to spill.  
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STR   Short tandem repeat (STR) analysis is a molecular biology method used to compare specific loci on DNA from two or more samples.  
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Electric Field   A region around a charged particle or object within which a force would be exerted on other charged particles or objects.  
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Inclusion   A body or particle recognizably distinct from the substance in which it is embedded.  
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Microliter   One millionth of a liter.  
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Human Genome   The human genome is the complete set of genetic information for humans. This information is located as DNA sequences within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual mitochondria.  
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Blunt Cut    
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Buffer   A solution that resists changes in pH when acid or alkali is added to it. Buffers typically involve a weak acid or alkali together with one of its salts.  
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Recombinant DNA   DNA that has been formed artificially by combining constituents from different organisms.  
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DNA Polymerase   The enzyme responsible for DNA replication  
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Gene   (in informal use) A unit of heredity that is transferred from a parent to offspring and is held to determine some characteristic of the offspring.  
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Digital Micropipet    
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Staggered Cut   The cleavage of two opposite strands of duplex DNA at points near one another.  
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Agarose Gel   Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and or size.  
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Inconclusive   Not leading to a firm conclusion; not ending doubt or dispute.  
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Fingerprint   An impression or mark made on a surface by a person's fingertip, esp. as used for identifying individuals from the unique pattern of whorls and lines.  
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Luminol   Luminol is a versatile chemical that exhibits chemiluminescence, with a striking blue glow, when mixed with an appropriate oxidizing agent.  
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PCR   Phosphocreatine, also known as creatine phosphate or PCr, is a phosphorylated creatine molecule that serves as a rapidly mobilizable reserve of high-energy phosphates in skeletal muscle and the brain.  
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Restriction Site   The specific sites at which a restriction enzyme will cleave DNA  
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Gel Electrophoresis   Gel electrophoresis is a method for separation and analysis of macromolecules and their fragments, based on their size and charge.  
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Evidence   The available body of facts or information indicating whether a belief or proposition is true or valid.  
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