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FNA/Cytology
Clin Path
| Question | Answer |
|---|---|
| Neutrophils, WBC's and macrophage are a sign of ___ | Inflammation |
| Cytology are done to differentiate ___ from ___ | Inflammation from neoplasia |
| Histopathology: | evaluates cellular architecture |
| Cytology: | evaluates cells individually/groups |
| ___ or ___ are used to collect swab cytology samples | Sterile cotton or rayon cells |
| ___ swabs are used for anarobic samples | Rayon swabs |
| Vaginal swabs are taken from ____ to ____ in the vagina | cranial to urethral orifice |
| ____ and ____Cells exfoliated from the vaginal wall are passed from the _____ | *neutrophils and epithelia *uterus |
| ___ are the only cytology's that are heat fixed | ear cytology |
| Scraping smears are useful for collecting cells from ___ but only contain ____ | *firm lesions *superficial |
| ___ shaped eosinaphils are only seen in felines | rod |
| ___ cytology's are prepared from external lesions from living animals or tissue samples | Imprint |
| Scraping procedure: | Hold scalpel blade perpendicular to sample and scrape several times, transfer to slide and spread like peanut butter |
| Imprint procedure: | remove blood/tissue fluid by blotting, make multiple imprints on several slides |
| Fine needle biopsy's can be a ____ or ____ procedure | Aspiration or non-aspiration |
| FNA use ___g needles and ___ml syringe | *21-25g needle *3-20ml syringe |
| The softer the aspiration tissue the ___ the needle and syringe | smaller |
| When redirecting the needle Constants ______ is necessary when using the FNA technique | negative pressure |
| ___g needle is moved through the same tract ___ times and repeated ___ times for a NON- aspirate technique. | *22G needle * 5-6 times *2-3 times |
| No ____ is used during the Non-aspirate technique. A ___ml syringe is used | *negative pressure *10 ml |
| ____ biopsy's are not ____ before procedure and are used for cytologic /histopathologic exams. | *Tisuue Biopsy's *scrubbed |
| Tissue samples for Histo are fixed in ___, the fixation of choice | *10 % formalin |
| Histo sample slabs are to be no more than ___ wide and fixed in formalin at a rate of ___ times the specimen's volume | *1 cm wide *1o times |
| Endoscopy biopsy's are flushed with ___ from tip of endoscopy | sterile saline |
| Wedge Biopsy's are taken from a ____ zone to ___ tissue | *transition *normal |
| Wedge biopsy's are placed on a ___ with the ___ facing up and the ___ facing down | * splint *superficial tissue *deep tissue |
| Most common punch biopsy's are _, _, and _mm. __ is the only one that doesn't require sutures | *4-6-8 *4mm |
| Centesis: | Introduction of a needle into any body cavity/organ to remove fluid |
| Synovial, cerbrospinal, and adequeous fluid all require ____ for collection | General anesthesia |
| Before collecting peritoneal and pleural fluid ____ prepare the site and equipment | aseptically |
| __g needle and __ml syringe is the most common used needle for centesis | *21g needle *60ml syringe |
| Needle is introduced into the ____ abdomen ___of the midline __cm caudal to umbilicus for abdominocentesis | *ventral *right *1-2cm |
| Exudate: | Thick with wbc's, rbc's, puss |
| Transudate: | clear or staw colored, primarily protein and H20 |
| peripheral blood contamination and recent hemorrhage result in ___ supernatant and __ sediment | *clear *red |
| Recent hemolysis results in ___ supernatant | reddish |
| 2 types of Transtracheal/bronchial are: | percutaneous or Orotracheal |
| insertion of needle into trachea through crycothyroid to collect fluid: | Percutaneous |
| Placing ET tube to collect fluid via catheter: | Orotracheal |
| Bronchoalveolar lavage is used to collect sample from the _____ | lower respiratory tract |
| Smear prep for solid masses: | Compression, combination, starfish |
| Fluid sample are collected into ___ tubes | *EDTA tubes |
| ___ Smears are similar to a blood smear except the slide in raised ___ through the smear and ___ smears are identical to blood smears | *Line smear *3/4 through *Wedge |
| Preferred fixative for cytology is ______ and should remain in fixative for __min | *95% methanol *2-5 |
| Malignancy must meet ___ or more nuclear criteria | 3 |
| Primary types of tumors: | Epithelial, Mesenchymal, round cell |
| Epithelial tumor: | Aka carcinoma or adeoncarcinoma, highly cellular in clumps |
| Mesenchymal tumors: | Aka sarcoma, less cellular/wispy spindles |
| Round cell tumors: | Histocytoma, lymphoma, mast cell, plasma cell, and melanoma |
| Neoplastic cells that spread from other body tissues to the lymph nodes: | Metastasis |
| malignant neoplasia of the lymph nodes is known as ___ | lymphoma |
| Gram negative robs are normal flora of the ___ | GI tract |
| The largest cells seen in a vaginal cytology are | superficial cells |
| fully cornified cells are ____ | anuclear |
| vaginal cytology's are a ___ assay | ENDOCRINE |
| Cells with a polygonal shape and smal N:C ratio: | Large intermediate |
| round/oval cell with prominent nuclei | small intermediate |
| RBC's are seen in large numbers during ____ | Proestrus |
| Neutrophils are in large numbers during____and absent during____ | *diestrus *estrus |
| bacteria is seen in every stage of estrous but especially during ___ | Estrus |
| Anestrus cells seen: | Parabasal, neutrophils (NO rbc'S) |
| Cells seen in Proestrus: | lrg int, superficail, RBC's and neutrophils |
| Proestrus can last __ wks | 2-3 |
| ___ stage is when breeding will occur | Estrus |
| Cells seen in Estrus: | anuclear (superficial)minimal Rbc's |
| cells seen in Diestrus: | parabasal, intermediate, abundant debris |
| Inflammation resulting in a pinkish-milky discharge and large numbers of neutrophils: | Vaginitis/Metritis |
| equipmemt and supplies used for semen should be warmed to ___deg F | 98.6 |
| 3 portions of ejaculate: | sperm free, sperm rich and sperm poor |
| 3 sections of a sperm: | head, mid piece, tail |
Created by:
brit_2112
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