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Restriction enzymes:
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The biological role of restriction enzymes is to:
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Chp 9MC
DNA-Based Information Technologies
| Question | Answer |
|---|---|
| Restriction enzymes: | are sequence-specific DNA endonucleases. |
| The biological role of restriction enzymes is to: | degrade foreign DNA that enters a bacterium. |
| The size of the DNA region specifically recognized by type II restriction enzymes is typically: | 4 to 6 base pairs. |
| Which of the following statements about type II restriction enzymes is FALSE? | They cleave and ligate DNA. |
| Which of the following statements about type II restriction enzymes is TRUE? | A) Many make staggered cuts w/in their recognition seq. B) Some cut DNA to generate blunt ends. C) They are part of a bacterial defense system in which foreign DNA is cleaved. D) They cleave DNA only at recognition seq specific to a given restric. enzy |
| Certain restriction enzymes produce cohesive (sticky) ends. This means that they: | make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding. |
| In the laboratory, recombinant plasmids are commonly introduced into bacterial cells by: | transformation – heat shock of the cells incubated with plasmid DNA in the presence of CaCl2. |
| The E. coli recombinant plasmid pBR322 has been widely utilized in genetic engineering experiments. pBR322 has all of the following features EXCEPT: | a number of palindromic sequences near the EcoRI site, which permit the plasmid to assume a conformation that protects newly inserted DNA from nuclease degradation. |
| The E. coli recombinant plasmid pBR322 has been widely utilized in genetic engineering experiments. pBR322 has all of the following features: | A) a # of conveniently located recog. sites for restrict. Es B) a rep. origin, which permits it to replicate autonomously. C) resist. to 2 diff antibiotics, rapid screening for recombinant plasmids containing foreign DNA. D) small overall size, which |
| Which of the following statements regarding plasmid cloning vectors is CORRECT? | The copy number of plasmids may vary from a few to several hundred. |
| Which of the following statements regarding plasmid cloning vectors is INCORRECT? | A) Circular plasmids do not require an origin of replication to be propagated in E. coli. B) Foreign DNA fragments up to 45,000 base pairs can be cloned in a typical plasmid. C) Plasmids do not need to contain genes that confer resistance to antibiotics |
| A convenient cloning vector with which to introduce foreign DNA into E. coli is a(n): | plasmid. |
| In genetic engineering, in vitro packaging is used to: | incorporate recombinant DNA into infectious bacteriophage particles. |
| Which of the following does NOT apply to the construction or use of a DNA library? | Genomic libraries are better for expressing gene products than cDNA libraries. |
| Which of the following DOES apply to the construction or use of a DNA library? | A) Determining the location of a particular DNA sequence in a DNA library requires a suitable hybridization probe C) Many segments of DNA from a cellular genome are cloned. D) Specialized DNA libraries can be made by cloning DNA copies of mRNAs. D) Th |
| The PCR reaction mixture does NOT include: | DNA ligase. |
| The PCR reaction mixture DOES include: | A) all four deoxynucleoside triphosphates. B) DNA containing the sequence to be amplified. C) heat-stable DNA polymerase. D) oligonucleotide primer(s). |
| Which of the following statements about the polymerase chain reaction (PCR) is FALSE? | DNA is amplified at many points within a cellular genome. |
| Which of the following statements about the polymerase chain reaction (PCR) is TRUE? | A) DNA amplified by PCR can be cloned. B) Newly synthesized DNA must be heat-denatured before the next round of DNA synthesis begins. C) The boundaries of the amplified DNA segment are determined by the synthetic oligonucleotides used to prime DNA synt |
| RFLP is a: | variation in DNA base sequence. |
| Current estimates indicate that humans have about ________ genes. | 30,000 |
| Which one of the following analytical techniques does NOT help illuminate a gene’s cellular function? | Southern blotting |
| Which one of the following analytical techniques DOES help illuminate a gene’s cellular function? | A) DNA microarray analysis B) Protein chip analysis C) Two-dimensional gel electrophoresis D) Two-hybrid analysis |
| The technique known as two hybrid analysis for detecting interacting gene products depend on: | stimulation of trasncription by interaction of two Gal4p domains via fused protein sequences. |
| A common cloning strategy for introducing foreign genes into plants with Agrobacterium employs all the following features EXCEPT: | a shuttle vector with 25 bp T-DNA repeats flanking the foreign gene of choice. |
| A common cloning strategy for introducing foreign genes into plants with Agrobacterium employs all the following features: | A) a selectable antibiotic marker such as kanamycin resistance. B) a Ti plasmid lacking its T-DNA segment. C) active vir gene products from the altered Ti plasmid. D) an ability to induce crown gall formation in infected leaves. |
Created by:
chemgeek1986