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Chp 9MC

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Created by: chemgeek1986

Question	Answer
Restriction enzymes:	are sequence-specific DNA endonucleases.
The biological role of restriction enzymes is to:	degrade foreign DNA that enters a bacterium.
The size of the DNA region specifically recognized by type II restriction enzymes is typically:	4 to 6 base pairs.
Which of the following statements about type II restriction enzymes is FALSE?	They cleave and ligate DNA.
Which of the following statements about type II restriction enzymes is TRUE?	A) Many make staggered cuts w/in their recognition seq. B) Some cut DNA to generate blunt ends. C) They are part of a bacterial defense system in which foreign DNA is cleaved. D) They cleave DNA only at recognition seq specific to a given restric. enzy
Certain restriction enzymes produce cohesive (sticky) ends. This means that they:	make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding.
In the laboratory, recombinant plasmids are commonly introduced into bacterial cells by:	transformation – heat shock of the cells incubated with plasmid DNA in the presence of CaCl2.
The E. coli recombinant plasmid pBR322 has been widely utilized in genetic engineering experiments. pBR322 has all of the following features EXCEPT:	a number of palindromic sequences near the EcoRI site, which permit the plasmid to assume a conformation that protects newly inserted DNA from nuclease degradation.
The E. coli recombinant plasmid pBR322 has been widely utilized in genetic engineering experiments. pBR322 has all of the following features:	A) a # of conveniently located recog. sites for restrict. Es B) a rep. origin, which permits it to replicate autonomously. C) resist. to 2 diff antibiotics, rapid screening for recombinant plasmids containing foreign DNA. D) small overall size, which
Which of the following statements regarding plasmid cloning vectors is CORRECT?	The copy number of plasmids may vary from a few to several hundred.
Which of the following statements regarding plasmid cloning vectors is INCORRECT?	A) Circular plasmids do not require an origin of replication to be propagated in E. coli. B) Foreign DNA fragments up to 45,000 base pairs can be cloned in a typical plasmid. C) Plasmids do not need to contain genes that confer resistance to antibiotics
A convenient cloning vector with which to introduce foreign DNA into E. coli is a(n):	plasmid.
In genetic engineering, in vitro packaging is used to:	incorporate recombinant DNA into infectious bacteriophage particles.
Which of the following does NOT apply to the construction or use of a DNA library?	Genomic libraries are better for expressing gene products than cDNA libraries.
Which of the following DOES apply to the construction or use of a DNA library?	A) Determining the location of a particular DNA sequence in a DNA library requires a suitable hybridization probe C) Many segments of DNA from a cellular genome are cloned. D) Specialized DNA libraries can be made by cloning DNA copies of mRNAs. D) Th
The PCR reaction mixture does NOT include:	DNA ligase.
The PCR reaction mixture DOES include:	A) all four deoxynucleoside triphosphates. B) DNA containing the sequence to be amplified. C) heat-stable DNA polymerase. D) oligonucleotide primer(s).
Which of the following statements about the polymerase chain reaction (PCR) is FALSE?	DNA is amplified at many points within a cellular genome.
Which of the following statements about the polymerase chain reaction (PCR) is TRUE?	A) DNA amplified by PCR can be cloned. B) Newly synthesized DNA must be heat-denatured before the next round of DNA synthesis begins. C) The boundaries of the amplified DNA segment are determined by the synthetic oligonucleotides used to prime DNA synt
RFLP is a:	variation in DNA base sequence.
Current estimates indicate that humans have about ________ genes.	30,000
Which one of the following analytical techniques does NOT help illuminate a gene’s cellular function?	Southern blotting
Which one of the following analytical techniques DOES help illuminate a gene’s cellular function?	A) DNA microarray analysis B) Protein chip analysis C) Two-dimensional gel electrophoresis D) Two-hybrid analysis
The technique known as two hybrid analysis for detecting interacting gene products depend on:	stimulation of trasncription by interaction of two Gal4p domains via fused protein sequences.
A common cloning strategy for introducing foreign genes into plants with Agrobacterium employs all the following features EXCEPT:	a shuttle vector with 25 bp T-DNA repeats flanking the foreign gene of choice.
A common cloning strategy for introducing foreign genes into plants with Agrobacterium employs all the following features:	A) a selectable antibiotic marker such as kanamycin resistance. B) a Ti plasmid lacking its T-DNA segment. C) active vir gene products from the altered Ti plasmid. D) an ability to induce crown gall formation in infected leaves.