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Neuro Research Metho
Ch 5
| Question | Answer |
|---|---|
| experimental types | determine/block/mimic/ measure |
| lesion | wound or injury/test theories about areas |
| substantia nigra | parkinson's |
| ablation | remove portion of brain |
| aspiration | suck out (cortex) |
| surgical | cut out |
| electrolytic | tissue destroyed/pin into area to eliminate |
| radio frequency | heat induced damage |
| electrolytic | current |
| neurotoxic | 6OHDA/ Kainate |
| temporary inactivation | -caine |
| physical | blunt force trauma |
| behavior analysis of lesions | Water maze/tasks/ dep on lesion |
| fixation | formalin= hard boiled egg |
| sectioning | microtome (meat slicer)/ vibratome/cryostat |
| nissl stain | cell body stain |
| immunocytochemistry | antibody/glia/chem on surface/GFAP/ChAT/TH |
| confocal microscopy | point illumination/spatial pinhole/eliminate out of focus light/3D |
| electron microscopy | tiny/vesicles etc/ scanning and transmission EM |
| neuronal tract tracing | markers used to trace pathways |
| anterograde | soma to terminal/PHA HSV |
| retrograde | to some/ fluorogold |
| microdialysis | NT levels in brain/ CSF = diff of other subs |
| macro e- recordings | EEG- large number |
| micro e- recordings | field potentials/single unit |
| autoradiography | x ray film/ nuclear emissions/ label region/ metabolism/ half-life changes |
| CT scan | x-ray 2D/ horizontal/ lesions/ abnorm |
| MRI | mag pulses and radio/ 3D |
| PET scan | radioactive tracers/ activity of structure |
| photostimulation | light-sens ion channels into cells/ specific wavelengths/ excite or inhibit |