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Neuro Research Metho

Ch 5

QuestionAnswer
experimental types determine/block/mimic/ measure
lesion wound or injury/test theories about areas
substantia nigra parkinson's
ablation remove portion of brain
aspiration suck out (cortex)
surgical cut out
electrolytic tissue destroyed/pin into area to eliminate
radio frequency heat induced damage
electrolytic current
neurotoxic 6OHDA/ Kainate
temporary inactivation -caine
physical blunt force trauma
behavior analysis of lesions Water maze/tasks/ dep on lesion
fixation formalin= hard boiled egg
sectioning microtome (meat slicer)/ vibratome/cryostat
nissl stain cell body stain
immunocytochemistry antibody/glia/chem on surface/GFAP/ChAT/TH
confocal microscopy point illumination/spatial pinhole/eliminate out of focus light/3D
electron microscopy tiny/vesicles etc/ scanning and transmission EM
neuronal tract tracing markers used to trace pathways
anterograde soma to terminal/PHA HSV
retrograde to some/ fluorogold
microdialysis NT levels in brain/ CSF = diff of other subs
macro e- recordings EEG- large number
micro e- recordings field potentials/single unit
autoradiography x ray film/ nuclear emissions/ label region/ metabolism/ half-life changes
CT scan x-ray 2D/ horizontal/ lesions/ abnorm
MRI mag pulses and radio/ 3D
PET scan radioactive tracers/ activity of structure
photostimulation light-sens ion channels into cells/ specific wavelengths/ excite or inhibit
Created by: olgakaczma
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