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IB 204
| Question | Answer |
|---|---|
| Which enzyme is responsible for unwinding DNA at the replication fork | Helicase |
| Which enzyme is responsible for relaxing supercoiling ahead of the DNA at the replication | Gyrase |
| Which enzyme is responsible for melting double stranded DNA exposing ssDNA for other proteins to bind | Initiator protein |
| Which enzyme is responsible for replacing RNA primers during DNA synthesis | DNA POL 3 |
| Which enzyme repairs gaps in the lagging strand following DNA synthesis? | Ligase |
| What step comes immediately before activity of DNA Pol I during DNA replication | Synthesis of DNA by POL 3 |
| What step is necessary for unwinding of the DNA by helicase | DNA gyrase relaxing supercoiling |
| During synthesis of DNA, Pol I catalyzes I. Removal of RNA primers II. Removal of DNA primers III. Joining of nicks between strands | I |
| Which of the following components are needed for PCR? I. DNA gyrase II. Taq DNA polymerase III. Primase | II |
| You receive a protocol to perform PCR, the extension step is for 30s. This would imply the length of the DNA fragment to be amplified is: | 1 kb |
| You receive a protocol to perform PCR, the annealing step is for 30s. This would imply the length of the DNA fragment to be amplified is | You CANNOT tell |
| The end replication occurs in eukaryotes because | Bacterial chromosomes are circular, whereas eukaryotic chromosomes are linear |
| Sequences at the end of eukaryotic chromosomes are referred to as | telomeres |
| MCM2-7 is licensed this means | It is positioned at origins of replication in preparation for replication |
| The minichromosome maintenance complex MCM2-7 plays an essential role in eukaryotic DNA replication by unwinding the DNA double helix. If a cell is in G2 phase, which of the following is most likely to be true: | MCM2-7 will be detached from origins of replication and DNA polymerase will be inactive. |
| If p53 is phosphorylated which of the following, can you infer is likely true: I. DNA is damaged. II. E2F is bound (inactive). III. CDK-Cyclin is active | i and II |
| If cyclin is not present and E2F is unbound, what can you infer: | The cell is cancerous, and DNA synthesis is activated |
| Which of the following types of genes are associated with a gain of function during development of Cancer? I. Tumor Suppressor II. DNA repair genes III. Oncogenes | III |
| Translate the following sequence: AGATGGGTTATCG | met-gly-Tyr |
| Comparing AGATGGGTTATCG and AGATGGGTATATCG What type of mutation has occurred to go from the first to the second sequence and what is the impact: | Insertion leading to a frameshift mutation |
| Comparing AGATGGGTTATCG and AGATGGGTTAACG What type of mutation has occurred to go from the first to the second sequence and what is the impact: | A substitution leading to a nonsense mutation |
| Comparing AGATGGGTTATCG and AGCATGGGTTATCG What type of mutation has occurred to go from the first to the second sequence and what is the impact: | Insertion that has no impact on protein sequence |
| If a nonsense mutation directly leads to cancer, what kind of gene can you infer it is likely to be in? | Tumor-suppressor gene |
| Sequence II is a mutated form of Sequence I. What type of mutation does Sequence II contain? I: “TATGCACTGGAAGA” II: “TATGCACTGAAAGA” | nonsense |
| How can dsDNA break repair result in mutations | Though insertions, deletions or chromosomal rearrangements during NHEJ |
| If repair of ROS mediated damage to DNA is not initiated, how can it lead to mutations during replication | Through non-canonical base pairing resulting in a substitution |
| Which of the following are activities of DNA Pol I? i. Exonuclease can remove RNA primers ii. DNA synthesis activity which can fill gaps from removal of RNA primers iii. Nick joining capacity that can seal gaps between two adjacent nucleotides | I and II |
| Which of the following are activities of DNA Pol III? i. Exonuclease can remove RNA primers ii. DNA synthesis capacity which adds DNA nucleotides to a growing strand iii. Nick joining capacity that can seal gaps between two adjacent nucleotides | II |
| Which of the following are activities of DNA ligase? i. Exonuclease can remove RNA primers ii. DNA synthesis capacity which adds DNA nucleotides to a growing strand iii. Nick joining capacity that can seal gaps between two adjacent nucleotides | III |
| Which protein has equivalent function to the MCM7-2 complex? | Helicase |
Created by:
hpokorny22
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