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Exam Two

Louisiana Tech University Dr. Kemeage BISC 130

QuestionAnswer
What is the plasma membrane described by "Fluid Mosaic Model"
What are the "Fluid Mosaic Model" components Phospholipids Cholesterol Integral membrane proteins Carbohydrates
Phospholipids Form bilayer
Cholesterol Stabilizes bilayer
Integral Membrane Proteins Amino acids interact with hydrophobic lipids to anchor it in the bilayer Many functions
Carbohydrates Glycolipids and Glycoproteins Create hydrophilic coating that attracts water
Explain the name "Fluid Mosaic Model" "Mosaic" because of many components "Fluid" because lipids and proteins are not covalently attached to each other - move laterally
Passive Transport Movement of materials that does not require energy
Diffusion Solutes move from high to lower concentration - "down" their concentration gradient
The plasma membrane is "Selectively permeable" - some atoms and molecules can move across it, but most can not
Facilitated Transport Channel proteins or carrier proteins allow for movement of solutes across plasma membrane, down their concentration gradient
Facts about channel proteins and carrier proteins Channels are faster than carriers Can be always open or gated by specific mechanisms Are very specific for the atom/molecule they transport Example - Aquaporin
Osmosis Movement of WATER through semipermeable membrane from an area of low solute concentration to an area of higher solute concentration
Due to aquaporins plasma membrane allows movement of water via osmosis in or out of the cell - movement of water depends on the amount of solutes in the surrounding concentration
What are the 3 types of solutions Hypotonic Hypertonic Isotonic
Hypotonic Solution Surroundings have fewer solutes than inside of cell Water moves into cell - cells swell, can burst Bad for animal cells, good for plant cells
Hypertonic Solution Surroundings have more solutes than inside of cells Water moves out of cell - cells shrivel Bad for cells
Isotonic Solutions Surroundings have same amount of solutes as inside of cell No net movement of water Good for animal cells, bad for plant cells
Active Transport Movement of materials that requires energy Moves materials against concentration gradient From low to high concentration
Bulk Transport Active transport of large structures/quantities 2 Types - endocytosis and exocytosis
Endocytosis Enters in vesicles/vacuoles Phagocytosis for large particle, pinocytosis for many small particles
Exocytosis Exits from vesicles
Energy The ability to do work 2 types - Potential and Kinetic
Potential Energy Stored Energy Includes chemical energy (energy in chemical bonds) *Breaking chemical bonds release energy
Kinetic Energy Energy of motion Includes heat
First Law of Thermodynamics Energy cannot be created or destroyed Only converted from one form to another
2 types of chemical reactions Exergonic and Endergonic
Exergonic reactions Products are at lower energy than reactants release energy
Endergonic reactions Products are at higher energy than reactants require input of energy
Facts about Exergonic and Endergonic reactions The two can be coupled Both need to overcome activation energy to proceed - (prevents reactions from spontaneously occurring) Heat energy can be used to overcome activation energy - (not feasible in cells
Catalyst Holds reactions and strains chemical bonds to lower activation energy Can facilitate exergonic or endergonic reactions Biological catalysts (most are protein) are called enzymes
Enzymes Catalyst Steps 1) Substrate(s) bind at enzyme's active site 2) Enzyme changes shape slightly to better fit substrates 3) Chemical reaction occurs 4) Product(s) released 5) Enzymes assumes original shape
Enzymes Catalyst step one Substrate(s) bind at enzyme's active site - form enzyme/substrate complex - enzymes are very specific for their substrates
Enzymes Catalyst step two Enzyme changes shape slightly to better fit substrates - "induced fit" - strains chemical bonds in substrate(s) lowering activation energy - for exergonic reactions, ambient heat energy is now enough to overcome activation energy
Enzymes Catalyst step five Enzymes assumes original shape - Not altered by reaction (free to work again)
Enzyme Inhibators Reduce an enzyme's function
Enzyme activators increase an enzyme's function
Some enzymes require cofactors and coenzymes to function
It takes a series of steps to build or break down a complex molecule. Each step carried out by a single enzyme called Metabolic Pathways
What are the two types of Metabolic pathways Anabolic Catabolic
Anabolic Pathway Build complex molecules Require energy input
Catabolic Pathway Break down complex molecules Release energy
ATP: Adenosine Triphosphate A nucleotide Has chemical potential energy (2 high-energy bonds btw 3 phosphate groups) Breaking them releases energy (exergonic) Coupled to endergonic reactions to provide the energy they need
ATP is the "what" of the cell The energy currency
ATP chemical equation ATP -> ADP + Pi -> AMP + Pi (-> represents energy)
Problems of ATP Unstable Energy is stored long term in other molecules, converted to ATP for use
Electrons have what kind of energy Potential energy
Loss of electrons Oxidation Lowered energy
Gain of electrons Reduction Higher energy
Oxidations and reductions are coupled Redox reactions
Along with electrons what else is transferred H+ ions
Electron carriers Some organic molecules readily gain or lose electrons Example - NAD+ (oxidized) and NADH (reduced)
Electrons can be at what kind of energy states High or low
Cellular Respiration The complete breakdown and oxidation of glucose to generate ATP Several Stages
Cellular Respiration chemical formula C6H12O6 + O2 -> CO2 + H2O + energy
Glycolysis 10 - enzyme metabolic pathway Glucose -> Pyruvate X2 (6-carbon) (3-carbon) ATP is spent in initial step, but a net amount is generated NAD+ reduced to NADH In Cytoplasm In Eukaryotes, all further steps in the mitochondria
Pyruvate Oxidation Pyruvate broken down, oxidized and attached to Coenzyme A (CoA) Releases CO2 Generates NADH Results in acetyl (CoA)
The Citric Acid Cycle Acetyl group (2 carbons) transferred from CoA to oxaloacetate (4 carbon) to form citrate (6 carbon) In many steps, citrate is broken down and oxidized until it is back to oxaloacetate CO2 X2 release ATP generated NADH generated FAD reduced to FADH2
Electron Transport Chain (ETC) NADH and FADH2 are oxidized back to NAD+ and FAD Energy from electrons used to pump H+ across the membrane *H+ cannot diffuse across the membrane
Step One of ETC NADH and FADH2 are oxidized back to NAD+ and FAD - their high-energy electrons passed through a series of other electron carriers -lose energy with each transfer - Finally transferred to O2 as low energy electrons
Step Two of ETC Energy from electrons used to pump H+ across the membrane - Active Transport - creates "protein gradient"
ATP Synthase A large multi-protein complex Spans the membrane Allows H+ to pass through down the concentration gradient Powers rotation of stalk, which generates ATP *Proton gradient is required for this to work *Most of ATP from cell respiration made here
Catabolism of Other Carbohydrates Broken monosaccharides, enter glycolysis
Catabolism of Proteins Broken to amino acids, enter glycolysis, pyruvate oxidation or citric acid cycle
Catabolism of Lipids/ Fatty Acids Broken into 2- carbon units, enter citric acid cycle
Categories of Organisms Heterotrophs and Autotrophs including Photoautotrophs (example: plants, some protists, some bacteria)
Chemical Formula for Photosynthesis CO2 + H2O + light -> C6H12O6 + O2
Photosynthesis 2 Parts: Light-Dependent Reactions and Light-Independent Reactions *In Eukaryotes, virtually all steps in chloroplasts
Light-Dependent Reactions (LDR) Require light
Light-Independent Reactions (LIR) Do not require light
What is visible light a type of Electromagnetic Radiation - Has specific wavelength - Different colors of visible light have different wavelengths
Light has what types of nature Wave and Particle -Particles of light are called photons - Carry Energy
Pigment Example: Chlorophylls: harness energy from photons for photosynthesis Example: Carotenoids: Dispose of excess energy
Hundreds of chlorophylls and carotenoids arranged into what light harvesting complexes - part of photsystems
The LDR Step One Photons strike pigments in photosystem II, excite electrons of chlorophylls to a higher energy state
The LDR step two Energy of excited electrons passed from one chlorophyll to another until it reaches the Reaction Center (RC) chlorophyll
The LDR step three RC chlorophyll delivers high-energy electrons through an electron transport chain - RC chlorophyll replaces lost electrons from H2O - H2O -> O2 + e-
The LDR step four High energy electrons passed through series pf carriers, lower energy with each passing - energy used to pump H+ across the membrane - Creates proton gradient - Used by ATP synthase to generate ATP
LDR step five Low energy electrons arrive at the photosystem I's RC chlorophyll
LDR step six Photons excite electrons in photosystems I's chlorophylls, energy moves to RC chlorophyll
LDR step seven High energy electrons in photosystem I used to reduce NADP+ to NADPH (electron carriers)
LDR overview H20 + photons -> O2 + ATP + NADPH
The LIR Takes place in the stroma (cytoplasm of chloroplast) Steps 1-3 are called the Calvin Cycle
LIR Step One An enzyme call RuBisCO performs carbon fixation by attaching CO2 to Ribulose Bisphosphate (5 carbons), creating 3- phosphoglyceric acid (3-PGA) X 2
LIR Step Two ATP and NADPH used to reduce 3-PGA to Glyceraldehyde
LIR Step Three ATP used to regenerate Ribulose Biphosphate from some G3P
LIR Step Four In cytoplasm, some G3P used to build amino acids, lipids, sugars - Converted to glucose through glycolysis - Same enzymes from cell respiration working in reverse
LIR Overview CO2 + ATP + NADPH -> C6H12O6
Ligands Released from signaling cells Bind to receptor proteins on target cells
4 types of Signaling Mechanisms Autocrine Direct Signaling Paracrine Endocrine
Autocrine Ligand binds to receptor on the signaling cell
Direct Signaling Ligand travels from cytoplasm to cytoplasm from signaling cell to target cell
Paracrine Ligand binds to receptors on nearby cells
Endocrine Ligand binds to receptor on distant cells Stable ligand ensures it can travel across the body
2 types of receptors intracellular receptor Cell surface receptors
Intracellular Receptors receptor protein in cytoplasm of cell ligands for these receptors must be small and hydrophobic to diffuse across the membrane Example - steroid hormones
Cell surface receptors Receptor protein on surface of cell Anchored in plasma membrane Must possess 3 Distinct membranes: Extracellular Domain Cytoplasmic Domain Transmembrane Domain
Extracellular Domain Exposed to outside of cell Binds ligand
Cytoplasmic Domain Exposed to cytoplasm
Transmembrane Domain Spans membrane
Cell Surface Receptors example Small molecules pr proteins *Ligands for these receptors are water-soluble
Signal Transduction Binding of ligand to change in shape of receptor (behaves differently) Example: an intracellular receptor may enter nucleus, causes genes to be turned on/off Example: May lead to release/creation of a second messenger
Phosphorylation Modification of protein by the addition of a phosphate group from ATP Catalyzed by a class of enzymes called kinases
Dephosphorylation Removal of a phosphate group from a protein Catalyzed by a class of enzymes called phosphates
Function of a protein can be switched on/off depending on its phosphorylation site
Signal transduction can ultimately result in Altered cell behavior cell growth/division Apoptosis
Single celled yeasts secrete a "mating factor" ligand to find yeasts cells to mate with Example: Quorum sensing - released ligands (autoinducers) communicate cell density to other bacteria -some bacteria can form biofilms, but this requires high cell density
Quorum sensing low ligand density
Ligand diffuses - no response High ligand density
Signaling pathway activated formation of biofilms
Aquaporin: a channel protein that allows water through the membrane at a very high rate
Carrier protein a membrane protein that moves a substance across the plasma membrane by changing its own shape
Channel protein a membrane protein that allows a substance to pass through its hollow core across the plasma membrane
Endocytosis type of active transport that moves substances into a cell
Exocytosis a type of active transport that moves substances out of a cell
Glycolipid: combination of carbohydrates and lipids
Glycoprotein a combination of carbohydrates and proteins
Solute a substance that is dissolved in a liquid to form a solution
Coenzyme: a small organic molecule that is required for optimal enzyme activity
Cofactor: an inorganic ion that is required for optimal enzyme activity
Metabolism: all of the chemical reactions that take place inside cells, including anabolic and catabolic pathways
Autotroph: an organism that gets carbon from inorganic sources
Carbon fixation the process of converting inorganic CO2 gas into organic compounds
Heterotroph an organism that consumes organic substances or other organisms for carbon
Photoautotroph an organism capable of producing its own organic compounds from inorganic carbon and energy from sunlight
Pigment a molecule that is capable of absorbing certain wavelengths of light and reflecting others
Apoptosis: programmed cell death
Biofilm a matrix of proteins and sugars that protects colonies of pathogenic bacteria from the host immune system
Ligand: a molecule produced by a signaling cell that binds with a specific receptor, delivering a signal in the process
Second messenger a small, non-protein molecule that propagates a signal within the cell after activation of a receptor causes its release
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