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collecting units of blood for transfusion 16 gauge needle
Regular sticks 21 gauge needle
Very small veins 23 gauge needles
List the parts common to all needles: Beveled point, shaft, hub
Using a 25 gauge needle with a 10ml evacuated tube may cause: hemolysis
How does the anticoag in the green stopper tube work: by inhibiting thrombin in the coag cascade
Name 3 anticoags that prevent clotting by binding calcium and the color coded stopper associated with them: sodium citrate- light blue EDTA- lavendar Sodium citrate-black
What is the purpose of sodium flouride in a gray stopper tube: Maintains glucose stability for 3 days
Why is EDTA the anticoag of choice for the CBC: Maintains cellular integrity better that the other anticoag: inhibits platelet clumping- doesn't interfere with routine staining procedures
What color tube that must always be completely filled: light blue
What is the purpose of tapping an evacuated tube containing dried anticoag before using it: loosen the powder from the tube for better results
Which of the following tubes will clot first: red, gold, or orange Orange
Under what circumstances should the amount of anticoag in a light blue stopper tube be decreased: Collecting on patients with polycythemia or hematocrit reading less than 55%
Why are royal blue stopper tubes used for collecting trace metal analyses: tubes are chemically cleaned and rubber stoppers are specially formulated to contain the lowest possible metal levels
When are winged infusion sets used in phlebtotomy: very small veins- children and geriatrics
Syringes are graduated in: mL and cc (milliliter/cubic centimeter)
When a blood pressure cuff is used as a tourniquet how should the pressure be adjusted: below the systolic bp but above the diastolic bp
List 2 precautions a phlebotomist would take when collecting a blood specimen from a patient with a latex allergy: use latex free tourniquet, gloves and bandages
List 2 antiseptics used in venipuncture and a state a situation when each is used: 70% isopropyl alcohol- to prevent contamination by normal skin bacteria during specimen collection iodine/chlorhexidene gluconate- additional sterility for collections such as arterial punctures and blood cultures
Lavendar stopper tubes and Hemoguard closures Anticoagulant- EDTA Purpose- chelates calcium Tests- CBC, ESR Inverted-x8
Pink hemoguard tubes Anticoag-EDTA Purpose-Blood banking Tests-T&C Inverted-x8 Section-Blood Bank
White hemoguard tubes Anticoag-EDTA and seperation gel Purpose-molecular diagnostics, MI panels and ammonia Inverted-x8
Light blue stopper tubes and hemoguard tubes Anticoag- sodium citrate Purpose- Binds calcium Tests- PT, PTT Blood/liquid ratio-9:1 Inverted-x3-4
Soybean trypsin inhibitor also in light blue tube used for fibrin degredation products
Black stopper tubes Anticoag- sodium citrate Purpose-Westergren sedimentation rate (automatic) blood/liquid ratio-4:1
Green stopper tubes and hemoguard tubes Anticoag- sodium heparin, ammonium heparin or lithium heparin Purpose- prevents clotting by inhibiting thrombin Tests- STAT lytes Interference-should not be used for hematology because it interferes with the Wrights stained blood smear
Light green Hemoguard/green & black stopper tubes Anticoag-lithium heparin and separation gel Purpose-Plasma determinations in chemistry Tests- Potassium Inverted-x8
Gray stopper tubes and hemoguard tubes Anticoag-potassium oxilate, EDTA Additives-sodium flouride, lithium iodoacetate Purpose-glucose preservatives Tests: Glucose testing/blood alcohol levels
Why are blood aclohol levels drawn in gray tubes with sodium flouride sodium flouride inhibits microbial growth which could produce alcohol as a metabolic end product
Royal blue stopper tubes Anticoags: None, sodium heparin Chemically clean tubes stoppers containing the lowest level of metals Tests: toxicology trace metal analysis, nutritional analysis invert 8x
Tan hemogaurd tubes Anticoag: (plastic) EDTA (glass) sodium heparin Tests: Lead levels invert 8x
Yellow stopper and hemoguard tubes 2 types: 1) Additive- ACD (rbc preservative) Tests- HLA phenotyping, paternity testing 2) Sterile yellow tubes Additive: SPS Purpose: Inhibits action of complement, phagocytes and some antibiotics Tests: Blood culture
Yellow/gray stopper tubes Orange hemogaurd tubes Additive: thrombin Purpose: results in faster clot formations (usually within 5 minutes) Tests: STAT serum chemistry and anticoag therapy
Red/gray stopper tubes Gold hemoguard tubes SST tubes-thixotropic gel Additives- silica, glass particles and celite Tests: chemistry 30 minute clot time then centrifuge
Red stopper and hemoguard tubes Glass- No additives Clot time is 60 minutes Tests: Blood bank, immunology, therauputic drug testing 2) Plastic- has silica for faster clotting
Order of the draw 1) Yellow (Sterile specimens 2) Glass red 3)Lt. blue 4) Red plastic 5) red/gray gold 6) Green 7) Lt. green 8) Lavender 9) Gray 10) Yellow gray, orange
When should you use syringes? On small, fragile veins
Why must you "pop" the syringe before using? Push air in and out to let out the vacuum
When should you use winged infusion sets? hand veins, peds and geriatrics, patients that move alot
What does a tourniquet do? impedes venous flow, doesnt stop arterial flow
What is aseptic cleansing? cleaning from the middle to the outside
Created by: amoodywife
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