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Ch 26 HW
Question | Answer |
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Genetic engineering, such as gene cloning and genome editing, is a type of ______ that can be used to change an organism or make desired biological products. | Biotechnology |
Scientists modify the genes of organisms using processes that are collectively referred to as ______. | genetic engineering |
Select all of the following that are reasons why a scientist may want to clone a gene? | to learn how the gene codes for a protein to use the gene for gene therapy to produce large quantities of the gene's protein product |
A segment of DNA containing genes from both mice and humans would be called ____________ DNA. | Recombinant |
An accessory ring of DNA in bacteria, often used as a vector in gene cloning, is a(n) | plasmid |
When humans use biological systems to produce a product, like insulin, they are using ______. | Biotechnology |
The enzyme that seals DNA into an opening created by restriction enzymes is called DNA _______ | ligase |
A bacterial genome may be modified using genetic ______ such that the organism produces a product that is useful to humans. | engineering |
Restriction enzymes are used to | cleave the vector DNA |
Biologists may choose to clone in order to produce large quantities of a product, such as insulin, or to use their clones to create a GMO. | genes |
The type of DNA that contains DNA from two or more different sources is called ______ DNA. | Recombinant |
How can DNA sequencing be applied? | as the foundation of the field of forensic biology development of disease-specific medications and treatments contributes to understanding evolution identification of specific alleles associated with disease |
Which terms are associated with the term plasmid? | bacterial DNA, vector |
What is the purpose of DNA sequencing? | It determines the exact sequence of nucleotides in a gene or piece of DNA. |
This enzyme is used during recombinant DNA production to seal openings created by restriction enzymes. | DNA ligase |
DNA sequencing methods usually involve dyes or radioactive tracers attached to each of the four ______ during DNA replication, and the results are then analyzed. | Nucleotides |
Enzymes used to cleave DNA at specific points during recombinant DNA production are called enzymes. | Restriction |
Polymerase chain reactions can create billions of segments of ______ in a test tube in just a few hours. | DNA |
DNA sequencing allows for researches to identify specific _____ that are associated with diseases, which allows for development of targeted medicines and treatments. | alleles |
Select the three basic steps that occur during the polymerase chain reaction. | annealing extension denaturation |
The technique used to analyze DNA fragments, whereby an electrical current is used to force DNA through a porous material, is called | gel electrophoresis |
Select all practical applications of DNA fingerprinting. | Identify the presence of a viral gene Identify criminals Identify poached wildlife Identify human remains |
The technique that uses DNA polymerase with heating/cooling cycles to produce millions of copies of a particular piece of DNA is called | the polymerase chain reaction |
Select all of the following applications of using CRISPR. | CRISPR can be used to cause a break in a gene, inactivating it, and allowing researchers to study its role in the body. CRISPR can be used to target specific genes and replace mutated nucleotides with correct ones. |
If a bacterium expresses a cloned gene, it is referred to as a(n) _______bacterium. | transgenic |
Arrange the steps in the polymerase chain reaction in order of occurrence, beginning with the first step at the top. | 1. DNA denaturation 2. DNA hybridization with primers 3. DNA extension by DNA polymerase |
Along with bacteria, _________ are being engineered to produce human proteins and antibodies as well as being engineered to be pest and herbicide resistant. | plants |
Gel electrophoresis is used to study fragments of _______, which separate according to their sizes on the gel. | DNA |
Drugs to treat which of the following may soon be available as a result of gene pharming? | cystic fibrosis blood diseases cancer |
DNA fingerprinting is used for | paternity testing. identifying microorganisms. detecting genetic diseases. tracing lineage of organisms. crime scene investigations. |
The main use of CRISPR-Cas9 technology is to | edit genes in their locations on the chromosome. |
The treatment of genetic disorders through the insertion of genetic materials into cells is called gene ______ | Therapy |
What are some of the uses of genetically modified plants? | reduced food waste stronger crops increased crop yield production of human proteins |
Using farm animals, researchers have plans for the development of drugs for the treatment of cancer, blood diseases, and cystic fibrosis by gene __________ | pharming |
Viruses, liposomes, and direct injections are techniques used to introduce a normal _______ into an individual who has a certain disease or genetic condition. | gene |
The insertion of genetic material into human cells for the treatment of a genetic disorder is called | gene therapy |
Arrange the events of ex vivo gene therapy for severe combined immunodeficiency (SCID), beginning with the first step at the top. | 1. bone marrow is removed from the patient's body 2. bone marrow cells are injected with a virus carrying a corrected gene 3. bone marrow cells are injected into the patient 4. normal bone marrow cells dived to produce normal blood cells. |
There is interest in injecting p53, a tumor suppressing gene, into cancer cells in order to | bring about apoptosis, or cell death, selectively. |
Which of the following may be used in order to introduce a gene into a cell? | direct injection liposomes genetically modified viruses |
RNA __________ occurs when a small piece of RNA is used to silence the expression of an allele. | Interference |
Genomics is the | study of the complete genetic sequences of organisms. |
A tumor suppressing gene known as p53 is being investigated as a type of gene therapy for cancer treatment based upon this gene's ability to bring about _________ in cells | Apoptosis |
True or false: The sequence of the human genome is now known. | true |
Proteomics is the study of all of the | proteins of a species. |
A gene's final product can be either the three types of RNA or a(n) | protein |
Bioinformatics uses _____ technologies to analyze genomic and proteomic information. | Computer |
Select all of the following that are direct applications of bioinformatics. | discovering the interplay of genes and proteins in our cells determining the function of genes comparison of our genome to model organisms |
A genetic profile of an individual shows mutations in their | genome |
The study of the complete collection of proteins produced by an organism is called . | proteomics |
The field of study that relies heavily on computer technologies to analyze genomic and proteomic data is called | Bioinformatics |
Mapping genes on chromosomes, knowing how cells and proteins interact, and comparing our genome to model organisms have all been enhanced because of using the computer-based science referred to as . | Bioinformatics |
An individual's genetic Blank______, which is established via DNA microarray, shows their genome, including mutations. | profile |