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Lecture 2

Chromatin Assembly

QuestionAnswer
What does a nucleosome consist of? a tetramer of H3-H4_2 and two dimers of H2A-H2B
How much DNA wraps around the nucleosome? 146 bp
How is chromatin organized? fibers link through a scaffold to form chromatin loops
Which form of packaged nucleosomes is transcriptionally active and relatively acetylated? euchromatin
Which form of nucleosomes are densely packed, relatively deacetylated, and transcriptionally inactive? heterochromatin
Which histone "links" DNA around each nucleosome? H1
When do nucleosomes assemble onto the chromatin S phase
What form does the (H3-H4)2 tetramer take in newly assembled nucleosomes? all old or all new
What form do H2A-H2B dimers take in newly assembled nucleosomes? old, new, or mixed
What are the two protein contributors to nucleosome formation? chaperones and remodeling enzymes
What are the Group I chaperones? CAF-1, HIR, ASF, RTT106, and NAP-1
What is the role of CAF-1? replication coupled nucleosome assembly; binds H3-H4 tetramer to PCNA
What is the role of HIR? replication independent assembly; binds H3-H4 tetramer
What is the role of ASF? histone donor; binds H3-H4 dimer and gives them to RTT106 and HIR
What is the role of RTT106? assembles H3-H4 tetramer during replication
What is the role of NAP-1? binds H2A-H2B, imports to nucleus
What are the Group II chaperones? FACT, ASF, and HIR
What is the role of FACT? binds tetramers to dimers; nucleosome remover; transcription elongation
What is the role of Group II ASF and HIR? bind tetramers to dimers
When are nucleosomes assembled? replication, transcription, repair/recombination, and differentiation
What is the role of BrdU? mark sites of new and active DNA synthesis
What are the four major types of nucleosome changes? contacts/remodeling, slippage/sliding, removal/displacement, exchange/replacement
What does Set1 do? methylates H3K4; associated with gene expression
What does Suvar-39 do? methylates H3K9; associated with gene repression
Created by: javaughnr
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