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Identify the additives in a serum separator tube.
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Describe the function of the additives in the SST
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Additives in tubes

QuestionAnswer
Identify the additives in a serum separator tube. Thixotropic Gel , clot activator
Describe the function of the additives in the SST Gel separates the clot from the serum. Preventing analytes which may leak out of the cells in the clot going into the serum and causing erroneous results. the clot activator enhances the clotting time
What colour is the tube top with the additive potassium oxalate and sodium fluoride as additives? Grey
What is the colour of the top of an EDTA? Lavender
What colour is the top of an ACD ( acid citrate dextrose) tube. Yellow
What colour is the top of a plasma separator and heparin. Light green
What colour is EDTA plasma separator tube? White stopper
What tube contain no additives and is used as discard tube? Clear stopper/ or red and grey dappled stopper
What antigoagulant is in the tube with the light blue stopper? Sodium citrate
Explain why it is important that a citrate is filled with the amount of blood stipulated on the label. The ratio of the blood to sodium citrate must be 9 parts blood to 1 part sodium citrate for the results to be accurate.
Identify the additive in a tube with a green stopper. It indicates that there is heparin in the tube. It may be lithium or sodium heparin
EDTA is the anticoagulant of choice for whole blood determinations in haematology because............. It inhibits platelet clumping. Maintains cellular integrity. Does not interfere with routine stains
List the correct order of draw for the closed system. Blood culture...sodium citrate/ light blue...red/gold/tube with or without additives-with and without gel. Heparin... EDTA...Grey...ACD
Give a reason why your 6th choice ( for order of draw) comes after the tube you listed as 5th on the order of draw. Because potassium oxalate damages the cell membrane of the red blood cell.
Give the additives to the following blood collection tubes and explain how each additive works: Yellow top tube • Acid citrate dextrose • Binds with the calcium in the blood to prevent coagulation cascade
The purpose of the tube additive sodium fluoride is to: Preserve the glucose.
Give 2 reasons why a light green top tube is an excellent choice for a stat (urgent) potassium? The blood does not need to clot - time is not wasted. ..The heparin does not interfere with the results. The gel prevents potassium leakage from the red blood cells into the plasma.
What is the purpose of the royal blue topped tube? Minimise trace element contamination.
Which of the following tubes contains an anticoagulant that works by binding calcium? *Light blue top *Green top *lavender top *grey top *red top *gold top Light blue top, Lavender top, grey top
To which department will a gold top tube go for analysis? Chemistry/serology
To which department will a light blue top tube go for analysis? Coagulation department in Haematology
To which department will a grey top tube go for analysis. Chemistry
To which department will a lavender top tube go for analysis? Haematology department
Give the additives to the following blood collection tubes and explain how each additive works: Lavender top tube • EDTA • Calcium in blood binds with the EDTA preventing the coagulation cascade
Give the additives to the following blood collection tubes and explain how each additive works: Grey top tube • Sodium fluoride acts as antiglycolytic (preserves the glucose for up to 3 days) • Potassium oxalate is an anticoagulant that binds with the calcium in the blood to prevent the coagulation cascade
Give the additives to the following blood collection tubes and explain how each additive works: Light blue top tube • Sodium citrate - its an anticoagulant • Prevents coagulation by combining with the calcium in the blood
Give the additives to the following blood collection tubes and explain how each additive works: Green top tube • Sodium or lithium heparin- it is an anticoagulant • Prevents coagulation by inhibiting thrombin in the coagulation cascade
Explain 2 benefits of the additive used in the purple/lavender tube • Maintains cellular integrity • Prevents platelets from clumping • Does not interfere with routine stains
Identify the tube that would be used when taking blood for toxicology and nutrient determinations, and explain why this tube is used for these tests Royal/dark blue stopper The tubes are trace element free
Name the tube that is known to cause more “ carry over” problems than any other tube EDTA tube
Which anticoagulant prevents coagulation by inhibiting thrombin formation Lithium Heparin
Identify the test that is most commonly done in the light blue stopper tube, AND to which laboratory department would it be sent for analysis • INR (International Normalization Ratio) • Coagulation department of haematology
How many times do we invert the tubes to ensure the blood and additive is adequately mixed? • 6-8 times
A blood sodium level may be taken using a green top tube containing sodium heparin. True or false? Give a reason for your answer • False • The sodium in the additive may falsely elevate the blood sodium result.
Explain the reason why blood cultures are first in the order of draw • In order to minimise the chance of microbial contamination
In the order of draw, the tube drawn after the heparin tubes is the: EDTA
To which laboratory department will the blood culture specimen go to for analysis? Microbiology
Give the reason why SST are drawn after citrate tubes Fill after coagulation tests because silica particles activate clotting and affect coagulation tests.
Give the reason why EDTA are drawn after SST Elevates Na ( esp with sod EDTA) and K levels chelates and decreases calcium and iron levels
Give a reason why the grey top tube is drawn after the lavender tube Filled after haematology because oxalate damages cell membranes and causes abnormal RBC morphology.
Created by: cisca
 

 



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