Additives in tubes
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Identify the additives in a serum separator tube. | show 🗑
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| show | Gel separates the clot from the serum. Preventing analytes which may leak out of the cells in the clot going into the serum and causing erroneous results. the clot activator enhances the clotting time
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| What colour is the tube top with the additive potassium oxalate and sodium fluoride as additives? | show 🗑
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| show | Lavender
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| What colour is the top of an ACD ( acid citrate dextrose) tube. | show 🗑
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| show | Light green
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| show | White stopper
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| What tube contain no additives and is used as discard tube? | show 🗑
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| What antigoagulant is in the tube with the light blue stopper? | show 🗑
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| Explain why it is important that a citrate is filled with the amount of blood stipulated on the label. | show 🗑
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| Identify the additive in a tube with a green stopper. | show 🗑
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| show | It inhibits platelet clumping. Maintains cellular integrity. Does not interfere with routine stains
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| show | Blood culture...sodium citrate/ light blue...red/gold/tube with or without additives-with and without gel. Heparin... EDTA...Grey...ACD
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| show | Because potassium oxalate damages the cell membrane of the red blood cell.
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| Give the additives to the following blood collection tubes and explain how each additive works: Yellow top tube | show 🗑
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| The purpose of the tube additive sodium fluoride is to: | show 🗑
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| show | The blood does not need to clot - time is not wasted. ..The heparin does not interfere with the results. The gel prevents potassium leakage from the red blood cells into the plasma.
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| show | Minimise trace element contamination.
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| show | Light blue top, Lavender top, grey top
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| show | Chemistry/serology
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| To which department will a light blue top tube go for analysis? | show 🗑
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| To which department will a grey top tube go for analysis. | show 🗑
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| show | Haematology department
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| Give the additives to the following blood collection tubes and explain how each additive works: Lavender top tube | show 🗑
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| show | • Sodium fluoride acts as antiglycolytic (preserves the glucose for up to 3 days)
• Potassium oxalate is an anticoagulant that binds with the calcium in the blood to prevent the coagulation cascade
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| show | • Sodium citrate - its an anticoagulant
• Prevents coagulation by combining with the calcium in the blood
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| Give the additives to the following blood collection tubes and explain how each additive works: Green top tube | show 🗑
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| show | • Maintains cellular integrity
• Prevents platelets from clumping
• Does not interfere with routine stains
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| show | Royal/dark blue stopper
The tubes are trace element free
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| show | EDTA tube
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| show | Lithium Heparin
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| Identify the test that is most commonly done in the light blue stopper tube, AND to which laboratory department would it be sent for analysis | show 🗑
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| How many times do we invert the tubes to ensure the blood and additive is adequately mixed? | show 🗑
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| show | • False
• The sodium in the additive may falsely elevate the blood sodium result.
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| show | • In order to minimise the chance of microbial contamination
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| In the order of draw, the tube drawn after the heparin tubes is the: | show 🗑
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| To which laboratory department will the blood culture specimen go to for analysis? | show 🗑
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| show | Fill after coagulation tests because silica particles activate clotting and affect coagulation tests.
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| Give the reason why EDTA are drawn after SST | show 🗑
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| show | Filled after haematology because oxalate damages cell membranes and causes abnormal RBC morphology.
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Created by:
cisca