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4TH QTR MICROBIOLOGY
Ms. Perkins- Microbiology- Test #2- Control of Microorganisms
Question | Answer |
---|---|
free flowing steam is about equal to | boiling |
which of the following is not a phenolic compound | glutaraldehyde |
why are ultraviolet rays lethal to bacteria | they damage the DNA or RNA of the microbe |
what process is used to destroy something of no value | incineration |
an agent that liberates gases for the purpose of destroying macroscopic life forms is a | fumigant |
the process of passing a liquid through a material with pores so small that bacteria cannot pass through is | filtration |
which of the following is the killing of all forms of life | sterilization |
the most effective method of a sterilization is | steam under pressure |
which of these is a chemical agent capable of killing viral organisms | virucide |
which of the following is used as a standard to measure the efficiency of chemical disinfectants | phenol |
which of these processes will destroy bacteria at 121 degrees celsius | steam under pressure |
which of these chemical agents is a member of the quaternary ammonium compound family | benzalkonium chloride |
which of these agents will kill bacteria | bactericide |
which of the following is not an effective disinfectant | soap |
the most commonly used agent for the process of sterilization is | heat |
the condition in which bacteria are prevented from growing and reproducing is | bacteriostasis |
which of the following will inhibit bacterial growth and reproduction | an antiseptic |
which of these is least effective in achieving a sterile environment | boiling |
which mechanical method of sanitation is likely to be used in the practice of mortuary hygiene | scrubbing |
if a chemical is more effective then phenol the coefficient is | a multiple of 1 |
for disinfection alcohol is most effective in a | seventy percent solution |
which of the following is not a factor in the action of a chemical disinfectant | time of day |
the polyatomic ion of hypochlorite is composed of chlorine | oxygen |
the killing or removal of all pathogenic microorganisms is | disinfection |
which of these chemical agents is often referred to as phenol | carbolic acid |
which of these is primarily bacteriostatic in its action | cold |
which of these chemical agents is described as a coal tar derivative | phenol |
what agent destroys yeasts and molds | fungicide |
embalming is both | disinfection and preservation |
sterilization is a process of completely | removing or destroying all life forms |
sterilization provides what level of microbial growth control | the highest |
what is the highest level of microbial growth control | sterilization |
what kills spores | sterilization |
disinfection is | the destruction of disease causing microorganisms or pathogens |
disease causing microorganisms | pathogens |
disinfection does not kill endospores but it does kill most | vegetative microorganisms |
antisepsis | bacteriostasis |
antisepsis is | the prevention or inhibiting of the growth of causative or pathogenic microorganisms |
which process can be used on living tissue because not as harsh as disinfectants | antiseptics |
the use of antiseptics does not result in the immediate killing of the organism, it simply | stops or greatly inhibits the growth of bacteria |
greek to stand still | sepsis |
physical / mechanical methods of control | scrubbing filtration sedimentation heat cold ultraviolet UV light |
chemical methods of control | disinfectant germicide bactericide fungicide virucide viricide insecticide larvicide fumigation |
methods of control | physical / mechanical and chemical |
scrubbing is | manual process by which microorganisms are removed |
scrubbing may be facilitated by the use of | soaps detergents or disinfectants |
filtration is | the use of biological filters to mechanically remove bacteria from liquids |
sedimentation is when | heavier particles settle to the bottom of the container |
heat | kills microorganisms |
heat kills microorganisms by | denaturing proteins |
proteins are denatured more easily when they are | wet |
one of the most reliable means of controlling the presence of microorganisms and their growth is | heat |
thermal death point | lowest temperature at which all microorganisms are killed in 10 minutes |
thermal death time | minimum time to kill all the bacteria at a given time |
decimal reduction time | time to kill 90 % of microbes that are present |
dry heat | higher temperatures can be achieved than boiling or free flowing steam |
incineration | organisms removed by burning |
incineration is form of | sterilization |
hot air | higher temperatures and higher exposure times needed when compared to moist heat |
an example of the use of hot air is | hot air oven |
hot air is better sometimes because it can | reach higher temperatures |
moist heat is | more effective at denaturing proteins than dry heat |
what is more effective at denaturing proteins than dry heat | moist heat |
compared to dry heat moist heat uses | lower temperatures and shorter exposure times |
usual exposure temperatures and times for moist heat are | 121 degrees celsius for 15 minutes |
which process uses lower temperatures and shorter exposure times | moist heat |
boiling is what process | disinfection |
water boils at what temperature | 100 degrees celsius |
how long do you need to boil things to kill vegetative bacteria most viruses and fungi | 10 minutes |
free flowing steam is | steam not under pressure |
free flowing steam can be used to both | disinfect or sterilize |
free flowing steam is usually used for | disinfection |
when and how is free flowing steam used to sterilize | fractional sterilization which is a 3 day process |
3 day process to kill both vegetative bacteria and endospores that germinate. 30 minute exposure time. about as effective as boiling since near the same temperature of 100 degrees celsius | fractional sterilization |
steam under pressure | autoclave pressure cooker |
autoclave is | higher pressure and higher temperatures |
autoclave pressure and temperature | 15 psi and 121 degrees celsius |
steam under pressure uses a shorter | exposure time |
autoclave time | 15 minutes |
autoclave is used for | sterilization |
steam under pressure gets rid of all | microbes |
cold is an | antisepsis technique |
cold | inhibits growth but does not kill all vegetative cells or endospores |
2 broad categories of chemicals | cide and static |
cide = | kill |
static = | inhibit growth |
germicide | destroys microorganisms |
bactericide | destroys bacteria |
fungicide | kills fungi and spores yeasts and molds |
virucide viricide | destructive to viruses |
insecticide | kills insects |
larvicide | kills insect larva |
fumigation | spraying chemicals or vapors into the air to kill insects or small animals |
fumigant | is the fumigation agent |
factors influencing the action of disinfectants | concentration type microbial load contact time temperature |
concentration of the disinfectant is | a factor that influences the action of disinfectants |
the type nature of the material to be disinfected is | a factor that influences the action of disinfectants |
the microbial load classification is | a factor that influences the action of disinfectants |
the contact time of disinfectant is | a factor that influences the action of disinfectants |
the temperature of disinfectant during exposure is | a factor that influences the action of disinfectants |
stronger concentration of a disinfectant is not always | better |
the longer the contact time of a disinfectant | the greater the kill |
what compounds are forbidden to be used in the mortuary | arsenic |
arsenic compounds are forbidden to be used | in the mortuary |
arsenic | AS |
ultraviolet UV light | inhibits growth by damaging genetic material |
direct UV light | will kill microorganisms |
desirable qualities of an ideal disinfectant | low toxicity to body tissues effective in the presence of organic matter stability / shelf life high penetrating power inexpensive |
which quality should be least important when looking at the qualities of an ideal disinfectant | cost |
when looking at qualities of an ideal disinfectant a main goal is | to not have to use more than gloves |
disinfectants suitable for mortuary procedures | halogens alcohols aldehydes phenolic compounds quaternary ammonium compounds |
types of halogens suitable for mortuary procedures | hypochlorites aka bleaches iodophores |
types of alcohols suitable for mortuary procedures | ethyl alcohol aka grain alcohol isopropyl alcohol aka rubbing alcohol |
types of aldehydes suitable for mortuary procedures | formalin aka formaldehyde solution glutaraldehyde dialdehyde aka cidex |
types of phenolic compounds suitable for mortuary procedures | phenol aka carbolic acid cresol aka lysol hexachlorophene aka phisoderm |
types of quaternary ammonium compounds suitable for mortuary procedures | benzalkonium chloride aka BAC zephiran chloride |
hypochlorites are | bleaches |
sodium hypochlorite = | NaOCl |
hypochlorite dilution | 1:10 |
1:10 dilution | hypochlorites |
hypochlorites are effective for | disinfecting and cleaning |
hypochlorites can be | irritating to skin and mucous membranes |
what is lethal if combined with ammonia | hypochlorites |
ammonia = | NH3 |
lethal is combined with formaldehyde | hypochlorites |
formaldehyde = | HCHO |
iodophores are | consisting of iodine |
iodine = | I |
iodophores are used for which process | disinfection |
a form of iodine that is an antiseptic | tincture of iodine |
alcohols are used for what process | disinfection |
alcohols are most effective in what solution | 70 % aqueous solution |
a negative to alcohols | volatile may evaporate before germicidal effect occurs |
aldehydes react with | proteins |
formalin is | aqueous formaldehyde solution |
aldehyde that is a known human carcinogen | formalin |
cidex | glutaraldehyde or dialdehyde |
glutaraldehyde | cold chemical sterilant |
phenolic compounds are | coal tar derivatives |
phenol aka | carbolic acid |
cresol aka | lysol |
hexachlorophene aka | phisoderm |
quaternary ammonium compounds are used as | disinfectants and antiseptics |
with quaternary ammonium compounds what increases strength | increased time |
quaternary ammonium compounds like benzalkonium chloride and zephiran chloride are neutralized in | hard water |
phenol coefficient test | the standard by which all disinfectants are measured |
if disinfectant is as effective as phenol the phenol coefficient number is | 1 |
is disinfectant is more effective that phenol the phenol coefficient number is | greater than 1 a multiple of 1 |
if disinfectant is less effective than phenol the phenol coefficient number is | less than 1 a fraction of 1 |
phenol coefficient number abbreviation | pc |