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What is the additive for the yellow tube?
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What is the additive for the study of anaerobic (without oxygen) bacteria in Blood Culture bottle?
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Phlebotomy Tubes
phlebotomy tube order, additives, and common tests
| Question | Answer |
|---|---|
| What is the additive for the yellow tube? | Acid Citrate Dextrose (ACD) Solution A or B |
| What is the additive for the study of anaerobic (without oxygen) bacteria in Blood Culture bottle? | Contain a nutrient rich broth to enable bacterial growth and an anticoagulant to prevent the blood from clotting called Sodium polyanethol Sulfonate (SPS) |
| What is the additive for the study of aerobic (with oxygen) bacteria in Blood Culture bottle? | Contain a nutrient rich broth to enable bacterial growth and an anticoagulant to prevent the blood from clotting called Sodium polyanethol sulfonate (SPS) |
| What is the additive in the black tube? | Sodium Citrate |
| What is the additive in the Light Blue tube? | Sodium Citrate |
| What is the additive in the Red-Clear top tube? | Does not contain an additive of any kind. |
| What is the additive in the Red top tube? | Silica clot activator |
| What is the additive in the Red/Black (tiger) top and Gold top tubes? | SST polymer gel separator tubes with clot activator |
| What is the additive in the Light Green top tube? | Lithium Heparin and gel for plasma separation |
| What is the additive in the Green top tube? | Sodium Heparin |
| What is the additive in the Green/Black striped top tube? | Polymer gel and Lithium Heparin |
| What is the additive in the Lavender top tube? | Ethylenediaminetetraacetic acid. Interior of the tube wall is coated with either EDTA K2 or EDTA K3 |
| What is the additive in the Pink top tube? | K3 EDTA |
| What is the additive in the Tan top tube? | • Sodium heparin (glass) • K2 EDTA (plastic) |
| What is the additive in the Royal Blue top tube? | • Sodium heparin • Na2 EDTA |
| What is the additive in the Gray top tube? | • Potassium oxalate/sodium floride • Sodium fluoride/Na2 EDTA |
| List common lab tests for which the Yellow top tube is used. | For whole blood tissue typing (DNA) & (HLA), paternity testing and organ donor testing. |
| List common lab tests for which the Blood Culture bottles are used. | Microbiology studies for fever of unknown origin. |
| List common lab tests for which the Black top tube is used. | Erythrocyte Sedimentation Rate (ESR) a non-specific measure of inflammation. |
| List common lab tests for which the Light Blue top tube is used. | APTT, INR, PT, Fibrinogen, D-Dimer |
| List common lab tests for which the Red-clear top tube is used. | Electrolytes, Infectious diseases HIV, STD’s. Drug levels: amikacin, amiodarone, amitriptyline, imipraimine and nortriptyline levels. Often used as a waste tube because it does not have any additives to cause cross contamination. |
| List common lab tests for which the Red top tube | Same tests as Red-clear but clots faster do not use as waste tube. |
| List common lab tests for which the Red/Black striped or Gold top tube is used. | ANA,ASO,basic metabolic panel (BMP),Calcium, comprehensive met.panel (CMP),Digoxin,lytes(Na+,K+,Cl,CO2),HIV,hormones,allergens,hepatitis,CK,troponin,myoglobin,lipids,hepatic,lithium,microsomal antibody, mononucleosis, PSA,renal function,thyroid testing. |
| List common lab tests for which the Light Green tube is used. | Hepatic (liver) function tests, electrolytes and markers of cardiac damage (troponin). Endocrine and metabolic disorders, therapeutic drug monitoring and toxicology, protein electrophoresis, lipid panels. Ammonia, HIV rapid Anti-body. |
| List common lab tests for which the Green top tube is used. | Stat K (potassium), lithium levels, electrolytes, ionized calcium Vitamin-B1 Thiamine |
| List common lab tests for which the Gray/Green striped top tube is used. | Glucose, cholesterol, triglycerides |
| List common lab tests for which the Lavender top tube is used. | CBC (Complete Blood Count); RBC count, WBC count & platelet count; WBC differential count; Hemoglobin & Hematocrit (H&H) determinations; ESR (erythrocyte sedimentation rate); sickle cell screening, A1-C |
| List the common lab tests for which the Pink top tube is used. | Blood donor screening, Type and Cross Match, blood bank determinations. |
| List the common lab tests for which the Tan top tube is used. | Lead levels |
| List the common lab tests for which the Royal Blue top tube is used. | Trace elements, nutritional chemistry, and toxicology Adult lead levels, arsenic, heavy metals, mercury and zinc. |
| List the common lab tests for which the Gray top tube is used. | Glucose determinations. |
| What special considerations are required when drawing blood for blood cultures? | Draw Anaerobic first unless using butterfly. Five preanalytical areas that can affect reliability of results. 1.timing of collection 2.number collected 3.amount of blood collected. 4.distribution between bottles. 5.disinfection of site. |
| What special considerations are required when drawing blood with a Light Blue top tube? | Do not over or under fill. If using butterfly draw waste tube first to prevent under filling tube. |
| What special considerations are required when drawing blood with a Red-Clear top tube? | Clots in 60 minutes. |
| What special considerations are required when drawing blood with a Red/Black top tube or Gold top tube? | Allow blood to clot upright for 30 minutes before centrifuging. |
| What special considerations are required when drawing blood with a Light Green top tube? | Do not use for lithium testing. Ice is needed to transport tube for ammonia levels. |
| What special considerations are required when drawing blood with a Green top tube? | Ionized Calcium needs to be transported in ice. |
| What special considerations are required when drawing blood with a Gray top tube? | Special anti-glycolytic additives stabilize glucose values for up to 24 hours at room temperature. |
| How many inversions should been done after drawing blood in a Yellow top tube? | 8 |
| How many inversions should been done after drawing blood in blood culture bottles? | 8 Collect 10 ml of blood using sterile technique into each bottle. Store/Transport @ Room Temperature within 24 hours of collection. For children under 12 yrs, 5 ml blood to 1 aerobic bottle. |
| How many inversions should been done after drawing blood in a Black top tube or Light Blue top tube? | 3-4 gently |
| How many inversions should been done after drawing blood in a Red top tube? | 5 |
| How many inversions should been done after drawing blood in a Red/Black striped top tube? | 5 |
| How many inversions should been done after drawing blood in a Light Green top tube? | 8 |
| How many inversions should been done after drawing blood in a Lavender top tube? | 8 |
| How many inversions should been done after drawing blood in a Pink top tube? | 8 |
| How many inversions should been done after drawing blood in a Tan top tube? | 8 |
| How many inversions should been done after drawing blood in a Royal Blue top tube? | 8 |
| How many inversions should been done after drawing blood in a Gray top tube? | 8 |
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