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Two safety concerns with heating devices
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Lab Safety Quiz 2

QuestionAnswer
Two safety concerns with heating devices Fire and burns
Examples of heating devices (5) Hotplate Heating Mantles Oil Baths Ovens Heating blocks
Combustible and flammable mixtures should never be heated unless ______. In a chemical fume hood
Three steps to do before using a heating device Check for auto shut off, Check condition of cord, Make sure it is well maintained
Vacuum Pump Parts (5) Have a belt guard, Have electric parts in good condition, Have a trap on the suction line, Have a cold trap Have a pan under the pump
To prevent explosions Flammable vapors must be released into a chemical fume hood, All electric equipment should be explosion proof,
Glassware should be checked for _________ and _______ before each use. cracks and scratches
All vacuum operations must be carried out where? Behind a table shield or fume hood
Vacuum equipment is subject to failure by __________. Implosion
Dewer vessels Have a vacuum between walls,
Desiccators Under partial vacuum, Need a perforated metal guard to deal with expansion and contraction
Centrifuges Responsibility (4) Proper loading/balancing, Operating at safe speeds, Safe stopping, Clean up
Things to check before using a centrifuge (3) Shielding against flyaways, Suction cups or brakes to prevent walking, Vibration won't affect equipment or experiments
If you have only one tube you need a ________ to ________ the centrifuge. blank, balance
Chemical Fume Hoods Keep flammable, toxic or odorous material from the individual
How to test a fume hood? Use a piece of paper to check airflow
Where do fume hoods release the air? Outside the building
Works only if the ______ is at the correct height. Sash
Do not block the fume hood _____. Baffles
All chemicals and equipment should be stored/used at least ___ inches behind the sash. 6
Keep the ____ completely lowered when not in use. Sash
Do not use the hood to ______ things. Store
Clean Bench Protects the _______ But not the ________ Experiment user
T/F Be sure to use slow careful movements so as to not disturb the laminar air flow. True
A clean bench should not be used with ________ materials. Toxic
T/F Clean benches have a HEPA filter. True
Biological Safety Cabinets Protects user, environment, work materials from aerosols
Class I BSC Personal and environmental protection But not sample protection (Like a fume hood, but with a HEPA filter)
Class II BSC Personal, environmental, and sample protection Safe for all biosafety levels (provided correct PPE for each level)
Class II A1 BSC Has how many HEPA filters? 2
Class II A1 BSC is different in four ways Air intake velocity, Amount of air recirculated, Determinate exhaust system, Pressure arrangements
Class III BSC for Ebola!! and the like BSL 3 and 4
Class III BSC special features (5) Glove ports, Double exhaust HEPA filters, Autoclave/Pass-through box, Chemical dunk tank, Independent exhaust system,
Before working in a BSC (3) List and place all materials in the BSC, Wear lab coat and suitable gloves, Adjust the stool height
Things to remember during (5) Move arms in and out slowly and perpendicular, Let cabinet sit for about one minute before working, Don't block grills, Work at least 4" away from the front grille, Work clean to dirty
T/F Extra supplies should be stored inside the BSC. False
T/F Even if a cabinet has been shut down it is always ready for immediate use. False
The UV lights don't clean things if they are ______. Dirty
After use disinfect the BSC with what? 70% Ethanol
Why should bleach not be used to clean our BSC? It causes corrosion
What type of BSC do we have? BSC Class II type A2
T/F The cabinet blower should not be running during decontamination. False, Keep that thing on
Final decontamination for the day should include a wipe down of four things. Work surface, Cabinet side, Cabinet back, Interior of glass
Small Spills in a BSC Can be handled immediately
Large spills means.... shut things down, close drain valve, pour decontamination stuff, wait 30 minutes,
WHAT DOES MSDS MEAN/?????? MATERIAL SAFETY DATA SHEET
WHERE CAN YOU FIND MSDS'S? THE INTERNET, OR I ASSUME SOME SORT OF BOOK IDK,
Government agency about safety with fire US Fire Administration
T/F All chemicals should be stored in alphabetical order to decrease confusion. False, that make hurt
Physical safety includes (7) Cuts, slips, trips, etc, Compressed gasses, Non-flammable cryogens, High pressure reactions Vacuum work, Ultra violet, visible, light Radio-frequency/Microwave
One way to minimize chemical exposure. Avoid skin contact with chemicals
Don't underestimate risk (3) Take special precautions when working with hazardous chemicals, Assume a mixture will be more toxic than its most toxic component, If toxicity is unknown, assume it is toxic
The best way to prevent exposure to aerosols is to have ______. Adequate ventilation
PELs Permissible exposure limits
TLVs Threshold limit value
"Routine exposure" Small lapses in protocol that are inconsequential but are then repeated
Constant _______ Vigilance
Before starting a procedure make a ____ specific to those chemicals, hazards, and equipment. Plan
Only use chemicals for which the available _______ system is appropriate. Ventilation
Before using a fume hood check to be sure ______. It is performing adequately.
The hood should be ______ unless work is being done inside. Closed
T/F If a hood is not in active use it can be turned off. False, the hood should be on as long as chemicals are in the hood.
If you need to leave an experiment: Leave the lights on, provide signs and labels explaining the experiment, provide proper containment
T/F Most chemicals can be disposed of down the drain. False. If unsure place it in a chemical/biological disposal
How long should skin be flushed after contact with chemical? 15 minutes
Don't eat in the lab
Sharps need to be disposed of in a ______. Tough plastic container
Examples of sharps blades, needles, lancets, sharp things
Reusable Vials, tubes, etc Autoclave bag > autoclave
Petri dishes, cultures, flasks, etc Biohazard container > autoclave
Pipette Tips Contaminated non-reusable tips Pipette disposal box > Glassware container
Pipette tip Contaminated reusable tip Tray...usually with bleach
Non contaminated tips you want to get rid of Glassware container > recycling
Glassware Contaminated intact glass Biohazard container
Glassware Contaminated broken glass Sharps container > biohazard waste
Glassware Non contaminated broken/useless glass Glassware container > recycling
Created by: tricec
 

 



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