Lab Safety Quiz 2
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| Two safety concerns with heating devices | Fire and burns
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| Examples of heating devices (5) | Hotplate
Heating Mantles
Oil Baths
Ovens
Heating blocks
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| Combustible and flammable mixtures should never be heated unless ______. | In a chemical fume hood
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| Three steps to do before using a heating device | Check for auto shut off,
Check condition of cord,
Make sure it is well maintained
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| Vacuum Pump Parts (5) | Have a belt guard,
Have electric parts in good condition,
Have a trap on the suction line,
Have a cold trap
Have a pan under the pump
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| To prevent explosions | Flammable vapors must be released into a chemical fume hood,
All electric equipment should be explosion proof,
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| Glassware should be checked for _________ and _______ before each use. | cracks and scratches
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| All vacuum operations must be carried out where? | Behind a table shield or fume hood
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| Vacuum equipment is subject to failure by __________. | Implosion
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| Dewer vessels | Have a vacuum between walls,
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| Desiccators | Under partial vacuum,
Need a perforated metal guard to deal with expansion and contraction
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| Centrifuges Responsibility (4) | Proper loading/balancing,
Operating at safe speeds,
Safe stopping,
Clean up
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| Things to check before using a centrifuge (3) | Shielding against flyaways,
Suction cups or brakes to prevent walking,
Vibration won't affect equipment or experiments
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| If you have only one tube you need a ________ to ________ the centrifuge. | blank,
balance
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| Chemical Fume Hoods | Keep flammable, toxic or odorous material from the individual
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| How to test a fume hood? | Use a piece of paper to check airflow
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| Where do fume hoods release the air? | Outside the building
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| Works only if the ______ is at the correct height. | Sash
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| Do not block the fume hood _____. | Baffles
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| All chemicals and equipment should be stored/used at least ___ inches behind the sash. | 6
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| Keep the ____ completely lowered when not in use. | Sash
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| Do not use the hood to ______ things. | Store
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| Clean Bench Protects the _______ But not the ________ | Experiment
user
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| T/F Be sure to use slow careful movements so as to not disturb the laminar air flow. | True
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| A clean bench should not be used with ________ materials. | Toxic
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| T/F Clean benches have a HEPA filter. | True
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| Biological Safety Cabinets | Protects user, environment, work materials from aerosols
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| Class I BSC | Personal and environmental protection
But not sample protection
(Like a fume hood, but with a HEPA filter)
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| Class II BSC | Personal, environmental, and sample protection
Safe for all biosafety levels (provided correct PPE for each level)
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| Class II A1 BSC Has how many HEPA filters? | 2
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| Class II A1 BSC is different in four ways | Air intake velocity,
Amount of air recirculated,
Determinate exhaust system,
Pressure arrangements
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| Class III BSC | for Ebola!! and the like
BSL 3 and 4
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| Class III BSC special features (5) | Glove ports,
Double exhaust HEPA filters,
Autoclave/Pass-through box,
Chemical dunk tank,
Independent exhaust system,
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| Before working in a BSC (3) | List and place all materials in the BSC,
Wear lab coat and suitable gloves,
Adjust the stool height
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| Things to remember during (5) | Move arms in and out slowly and perpendicular,
Let cabinet sit for about one minute before working,
Don't block grills,
Work at least 4" away from the front grille,
Work clean to dirty
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| T/F Extra supplies should be stored inside the BSC. | False
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| T/F Even if a cabinet has been shut down it is always ready for immediate use. | False
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| The UV lights don't clean things if they are ______. | Dirty
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| After use disinfect the BSC with what? | 70% Ethanol
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| Why should bleach not be used to clean our BSC? | It causes corrosion
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| What type of BSC do we have? | BSC Class II type A2
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| T/F The cabinet blower should not be running during decontamination. | False,
Keep that thing on
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| Final decontamination for the day should include a wipe down of four things. | Work surface,
Cabinet side,
Cabinet back,
Interior of glass
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| Small Spills in a BSC | Can be handled immediately
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| Large spills means.... | shut things down, close drain valve, pour decontamination stuff, wait 30 minutes,
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| WHAT DOES MSDS MEAN/?????? | MATERIAL SAFETY DATA SHEET
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| WHERE CAN YOU FIND MSDS'S? | THE INTERNET, OR I ASSUME SOME SORT OF BOOK IDK,
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| Government agency about safety with fire | US Fire Administration
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| T/F All chemicals should be stored in alphabetical order to decrease confusion. | False, that make hurt
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| Physical safety includes (7) | Cuts, slips, trips, etc,
Compressed gasses,
Non-flammable cryogens,
High pressure reactions
Vacuum work,
Ultra violet, visible, light
Radio-frequency/Microwave
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| One way to minimize chemical exposure. | Avoid skin contact with chemicals
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| Don't underestimate risk (3) | Take special precautions when working with hazardous chemicals,
Assume a mixture will be more toxic than its most toxic component,
If toxicity is unknown, assume it is toxic
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| The best way to prevent exposure to aerosols is to have ______. | Adequate ventilation
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| PELs | Permissible exposure limits
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| TLVs | Threshold limit value
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| "Routine exposure" | Small lapses in protocol that are inconsequential but are then repeated
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| Constant _______ | Vigilance
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| Before starting a procedure make a ____ specific to those chemicals, hazards, and equipment. | Plan
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| Only use chemicals for which the available _______ system is appropriate. | Ventilation
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| Before using a fume hood check to be sure ______. | It is performing adequately.
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| The hood should be ______ unless work is being done inside. | Closed
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| T/F If a hood is not in active use it can be turned off. | False, the hood should be on as long as chemicals are in the hood.
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| If you need to leave an experiment: | Leave the lights on, provide signs and labels explaining the experiment, provide proper containment
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| T/F Most chemicals can be disposed of down the drain. | False. If unsure place it in a chemical/biological disposal
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| How long should skin be flushed after contact with chemical? | 15 minutes
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| Don't eat in the lab |
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| Sharps need to be disposed of in a ______. | Tough plastic container
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| Examples of sharps | blades, needles, lancets, sharp things
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| Reusable Vials, tubes, etc | Autoclave bag > autoclave
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| Petri dishes, cultures, flasks, etc | Biohazard container > autoclave
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| Pipette Tips Contaminated non-reusable tips | Pipette disposal box > Glassware container
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| Pipette tip Contaminated reusable tip | Tray...usually with bleach
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| Non contaminated tips you want to get rid of | Glassware container > recycling
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| Glassware Contaminated intact glass | Biohazard container
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| Glassware Contaminated broken glass | Sharps container > biohazard waste
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| Glassware Non contaminated broken/useless glass | Glassware container > recycling
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Created by:
tricec