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The reaction catalyzed by reverse transcriptase.
A) DNA -> mRNA
B) mRNA -> cDNA
C) mRNA ->protein
D) DNA -> DNA
E) None
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Question
If you have inserted a gene in the Ti, the next step in genetic engineering is
A) Transformation of E. coli with Ti.
B) Splicing Ti into a plasmid.
C) Transformation of an animal cell.
D) Inserting Ti into Agrobacterium.
E) None of the above.
A) Transformation of E. coli with Ti.
B) Splicing Ti into a plasmid.
C) Transformation of an animal cell.
D) Inserting Ti into Agrobacterium.
E) None of the above.
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Micro unit 5 ch 9
unit 5 chapter 9
| Question | Answer |
|---|---|
| The reaction catalyzed by reverse transcriptase. A) DNA -> mRNA B) mRNA -> cDNA C) mRNA ->protein D) DNA -> DNA E) None | B |
| If you have inserted a gene in the Ti, the next step in genetic engineering is A) Transformation of E. coli with Ti. B) Splicing Ti into a plasmid. C) Transformation of an animal cell. D) Inserting Ti into Agrobacterium. E) None of the above. | D |
| A population of cells carrying a desired plasmid is called a A) Library. B) Clone. C) Vector. D) Southern blot. E) PCR. | B |
| Self-replicating DNA used to transmit a gene from one organism to another is a A) Library. B) Clone. C) Vector. D) Southern blot. E) PCR. | C |
| A technique used to identify bacteria carrying a specific gene is A) Southern blot. B) Shotgun sequencing. C) Transformation. D) Cloning. | A |
| Which of the following techniques is used to alter one amino acid in protein? A) Cloning B) PCR C) Restriction enzymes D) Selection E) Site-direct mutagenesis | E |
| A source of heat-stable DNA polymerase is A) Agrobacterium tumefaciens. B) Thermus aquaticus. C) Saccharomyces cerevisiae. D) Bacillus thuringiensis. E) Pseudomonas. | B |
| Which organism naturally possesses the Ti plasmid? A) Agrobacterium tumefaciens B) Thermus aquaticus C) Saccharomyces cervisiae D) Bacillus thuringiensis E) Pseudomonas | A |
| Which organism degrades PCBs and has been engineered to produce BT toxin? A) Agrobacterium tumefaciens B) Thermus aquaticus C) Saccharomyces cerevisiae D) Bacillus thuringiensis E) Pseudomonas | E |
| An example of gene therapy is A) Insertion of the insulin gene in E. coli. B) Insertion of the insulin gene in a mammalian cell culture. C) Insertion of the insulin gene in a diabetic person's pancreas cells. D) Injection of insulin into a diabeti | C |
| The purpose of using an antibiotic resistance gene on a plasmid used in genetic engineering makes A) Replica plating possible. B) Direct selection possible. C) The recombinant cell dangerous. D) The recombinant cell unable to survive. E) All of | B |
| A specific gene can be directly inserted into a cell by all of the following except A) Protoplast fusion. B) a gene gun. C) Microinjection. D) Electroporation. E) Agrobacterium. | A |
| PCR can be used to identify a bacterium because A) The DNA primers are specific. B) DNA polymerase will replicate DNA. C) DNA can be electrophoresed. D) All cells have DNA. E) None of the above. | A |
| Restriction enzymes are A) Bacterial enzymes that splice DNA. B) Bacterial enzymes that destroy phage DNA. C) Animal enzymes that splice RNA. D) Viral enzymes that destroy host DNA. | B |
| The necessary ingredients for DNA synthesis can be mixed together in a test tube. The DNA polymerase is from Thermus aquaticus and the template is from a human cell. The DNA synthesized would be most similar to A) Human DNA. B) T. aquaticus DNA. | A |
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