Micro unit 5 ch 9 Word Scramble

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Question	Answer
The reaction catalyzed by reverse transcriptase. A) DNA -> mRNA B) mRNA -> cDNA C) mRNA ->protein D) DNA -> DNA E) None	B
If you have inserted a gene in the Ti, the next step in genetic engineering is A) Transformation of E. coli with Ti. B) Splicing Ti into a plasmid. C) Transformation of an animal cell. D) Inserting Ti into Agrobacterium. E) None of the above.	D
A population of cells carrying a desired plasmid is called a A) Library. B) Clone. C) Vector. D) Southern blot. E) PCR.	B
Self-replicating DNA used to transmit a gene from one organism to another is a A) Library. B) Clone. C) Vector. D) Southern blot. E) PCR.	C
A technique used to identify bacteria carrying a specific gene is A) Southern blot. B) Shotgun sequencing. C) Transformation. D) Cloning.	A
Which of the following techniques is used to alter one amino acid in protein? A) Cloning B) PCR C) Restriction enzymes D) Selection E) Site-direct mutagenesis	E
A source of heat-stable DNA polymerase is A) Agrobacterium tumefaciens. B) Thermus aquaticus. C) Saccharomyces cerevisiae. D) Bacillus thuringiensis. E) Pseudomonas.	B
Which organism naturally possesses the Ti plasmid? A) Agrobacterium tumefaciens B) Thermus aquaticus C) Saccharomyces cervisiae D) Bacillus thuringiensis E) Pseudomonas	A
Which organism degrades PCBs and has been engineered to produce BT toxin? A) Agrobacterium tumefaciens B) Thermus aquaticus C) Saccharomyces cerevisiae D) Bacillus thuringiensis E) Pseudomonas	E
An example of gene therapy is A) Insertion of the insulin gene in E. coli. B) Insertion of the insulin gene in a mammalian cell culture. C) Insertion of the insulin gene in a diabetic person's pancreas cells. D) Injection of insulin into a diabeti	C
The purpose of using an antibiotic resistance gene on a plasmid used in genetic engineering makes A) Replica plating possible. B) Direct selection possible. C) The recombinant cell dangerous. D) The recombinant cell unable to survive. E) All of	B
A specific gene can be directly inserted into a cell by all of the following except A) Protoplast fusion. B) a gene gun. C) Microinjection. D) Electroporation. E) Agrobacterium.	A
PCR can be used to identify a bacterium because A) The DNA primers are specific. B) DNA polymerase will replicate DNA. C) DNA can be electrophoresed. D) All cells have DNA. E) None of the above.	A
Restriction enzymes are A) Bacterial enzymes that splice DNA. B) Bacterial enzymes that destroy phage DNA. C) Animal enzymes that splice RNA. D) Viral enzymes that destroy host DNA.	B
The necessary ingredients for DNA synthesis can be mixed together in a test tube. The DNA polymerase is from Thermus aquaticus and the template is from a human cell. The DNA synthesized would be most similar to A) Human DNA. B) T. aquaticus DNA.	A

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