Detection of antinuclear auto-antibodies
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| FITC | Fluorescein isothiocyanate
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| What induces autoimmune diseases? | Self-reacting antibodies, genetic disposition, hormones, aging, infections, trauma, drugs.
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| A hallmark of autoimmune diseases? | Presence of auto-reactive antibodies directed against antigen(s).
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| FITC | Fluorescein isothiocyanate
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| SLE | Systemic lupus erythematosus
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| A hallmark of autoimmune diseases? | Auto-reactive antibodies that target antigen(s), causing organ or system malfunction.
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| A standard method for detecting antinuclear autoantibodies? | Indirect immunofluorescence
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| Indirect immunofluorescence materials | Tissue sections from rat kidney or liver (antigen). Human serum as primary antibody. Fluorescence labeled or secondary antibody for detection.
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| What produces different staining patterns? | Antinuclear autoantibodies with different nuclear targets
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| Homogenous nuclear staining is caused by? | Targeting of DNA-nucleoprotein histone complexes
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| Peripheral staining of nuclear membrane is caused by? | Antibodies against DNA.
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