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Immunofluorescence
Detection of antinuclear auto-antibodies
Question | Answer |
---|---|
FITC | Fluorescein isothiocyanate |
What induces autoimmune diseases? | Self-reacting antibodies, genetic disposition, hormones, aging, infections, trauma, drugs. |
A hallmark of autoimmune diseases? | Presence of auto-reactive antibodies directed against antigen(s). |
FITC | Fluorescein isothiocyanate |
SLE | Systemic lupus erythematosus |
A hallmark of autoimmune diseases? | Auto-reactive antibodies that target antigen(s), causing organ or system malfunction. |
A standard method for detecting antinuclear autoantibodies? | Indirect immunofluorescence |
Indirect immunofluorescence materials | Tissue sections from rat kidney or liver (antigen). Human serum as primary antibody. Fluorescence labeled or secondary antibody for detection. |
What produces different staining patterns? | Antinuclear autoantibodies with different nuclear targets |
Homogenous nuclear staining is caused by? | Targeting of DNA-nucleoprotein histone complexes |
Peripheral staining of nuclear membrane is caused by? | Antibodies against DNA. |