Enzyme Kinetics (Michaelis Menten/Lineweaver Burke/Eadie-Hostee)
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| What is the Michaelis Menten equation? | v=Vmax [S] / [S] + Km
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| Define Vmax. | The rate of the reaction at saturation substrate concentrations for a given amount of enzyme.
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| The rate of the reaction at saturation substrate concentrations for a given amount of enzyme defines what concept? | Vmax
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| Define Km. | The substrate concentration that results in 50% Vmax.
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| The substrate concentration that results in 50% Vmax defines what? | Km
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| An inhibitor that binds to the active site and competes with the substrate for its occupation is called a _________ inhibitor. | Competitive inhibitor
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| An inhibitor that binds to, and takes an enzyme out of commission is called a ____________ inhibitor. | Noncompetitive inhibitor
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| Competitive inhibitors ____________ (increase / decrease) the apparent Km. | Increase
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| In a Lineweaver Burke plot, a competitive inhibitor will have _______________ (same / different) point on the x axis. | Different
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| In a Lineweaver Burke plot, a noncompetitive inhibitor will have _______________ (same / different) point on the x axis. | Same
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| In a Lineweaver Burke plot, a competitive inhibitor will have _______________ (same / different) point on the y axis. | Same
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| In a Lineweaver Burke plot, a noncompetitive inhibitor will have _______________ (same / different) point on the y axis. | Different
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| This type of inhibitor binds to the active site and is acted upon by the enzyme just as if it was a normal substrate. The enzyme uses its own catalytic machinery to inactivate itself. | Suicide inhibitor
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| Fluorouracil is what type of inhibitor? | Suicide inhibitor
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| The Y axis on the Lineweaver Burke plot indicates ___________. | 1/Vmax
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| The X axis on the Lineweaver Burke plot indicates ____________. | -1/Km
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| The Y axis on the Eadie-Hofstee plot indicates _________. | Vmax
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| The X axis on the Eadie-Hofstee plot indicates _________. | Vmax/Km
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| The slope of the Eadie-Hofstee plot indicates __________. | -Km
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| True or False: Both competitive and noncompetitive inhibition is reversible. | True
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| True or False: Both competitive and noncompetitive inhibition is irreversible. | False
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