The rate of the reaction at saturation substrate concentrations for a given amount of enzyme.
The rate of the reaction at saturation substrate concentrations for a given amount of enzyme defines what concept?
Vmax
Define Km.
The substrate concentration that results in 50% Vmax.
The substrate concentration that results in 50% Vmax defines what?
Km
An inhibitor that binds to the active site and competes with the substrate for its occupation is called a _________ inhibitor.
Competitive inhibitor
An inhibitor that binds to, and takes an enzyme out of commission is called a ____________ inhibitor.
Noncompetitive inhibitor
Competitive inhibitors ____________ (increase / decrease) the apparent Km.
Increase
In a Lineweaver Burke plot, a competitive inhibitor will have _______________ (same / different) point on the x axis.
Different
In a Lineweaver Burke plot, a noncompetitive inhibitor will have _______________ (same / different) point on the x axis.
Same
In a Lineweaver Burke plot, a competitive inhibitor will have _______________ (same / different) point on the y axis.
Same
In a Lineweaver Burke plot, a noncompetitive inhibitor will have _______________ (same / different) point on the y axis.
Different
This type of inhibitor binds to the active site and is acted upon by the enzyme just as if it was a normal substrate. The enzyme uses its own catalytic machinery to inactivate itself.
Suicide inhibitor
Fluorouracil is what type of inhibitor?
Suicide inhibitor
The Y axis on the Lineweaver Burke plot indicates ___________.
1/Vmax
The X axis on the Lineweaver Burke plot indicates ____________.
-1/Km
The Y axis on the Eadie-Hofstee plot indicates _________.
Vmax
The X axis on the Eadie-Hofstee plot indicates _________.
Vmax/Km
The slope of the Eadie-Hofstee plot indicates __________.
-Km
True or False: Both competitive and noncompetitive inhibition is reversible.
True
True or False: Both competitive and noncompetitive inhibition is irreversible.
Cardio Block2anatomy
