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biochem pathways
Question | Answer |
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aerobic glycolysis f/s/p | Functions: Produce energy and produce substrates for other anabolic pathways. Substrates: Glucose (or G-6-P),ADP,NAD+ Product: ATP, pyruvate/lactate, NADH--->if lactate is the product, NADH isn't the product |
aerobic glycolysis c/r/c/t | Control Enzymes: PFK-1 (phosphofructokinase-1) Regulation: Fructose 2,6-bisphosphate and ratio of ATP to ADP Compartment(s): Cytosol Tissues of interest: ALL OF THEM! |
tca f/s/p | Fx:-to produce energy (in the form of ATP) by the oxidation of foodstuffs! -Eight e- are donated by the acetyl group (3 nadh get 2 e-, fadh gets 2 e-) Subs: Acetyl CoA, H2O, NAD+, FAD, GDP, Pi Products: CO2, NADH, H+, FADH2, GTP |
tca c/r/c/t | Control Enzymes: -2 primary control enzymes: Isocitrate dehydrogenase and a-Ketoglutarate dehydrogenase -other- CAIK sounds so fucking mint Regulation: ATP/ADP ratio and the rate of ATP use Compartment(s): Mitochondrial matrix Tissues: all except rbc |
glycogen synthesis f/s/p | Fx: maintain blood glucose later(liver). glucose so you can use it for energy for contractions at a later time (muscle). Substrates: ATP, glucose, UTP Products: ADP, (glucose-6-phosphate is an intermediate), glycogen, pyrophosphate (PPi --> 2Pi), and U |
glycogen synthesis c/r/c/t | Control Enzyme: glycogen synthase Regulation: Insulin/Glucagon ratio and amount of glucose present (liver), epinephrine can also inhibit. Muscle insulin level (muscle). Compartment: cytosol tissues: liver and muscles |
glycogenolysis (glycogen degradation)f/s/p | Function: Provide blood glucose (liver). Provide glucose to produce energy for muscle contractions (muscle). Substrates: Glycogen, Free phosphates Products: free glucose and g-1-p or g-6-p |
glycogenolysis (glycogen degradation)c/r/c/t | Control: debrancher enzyme, glycogen phosphorylase Reg: G phosphorylase, also epi has an effect. (Phosphorylation activates control enzyme.) In muscle, GNL is regulated via activation of glycogen phosphorylase by [AMP], [Ca2+],presence of epi C: Cytosol |
pentose phosphate pathway function | Functions: To make NADPH (used for detoxification [ie. glutathione synthesis] and Reductive Biosynthesis [ie. fatty acid synthesis]) and Ribose 5 phosphate (nucleotide synthesis) . |
pentose phosphate pathway s/p | Substrates: Glucose-6-phosphate and NADP+ Products: NADPH, CO2, ribose-5-phosphate, fructose-6-phosphate, and glyceraldehyde-3-phosphate |
ppp pathways | There are two routes the pathway can take, oxidative (irreversible) and nonoxidative (reversible). |
ppp oxidative pathway | The first pathway is the oxidative pathway. This is where NADPH, ribulose 5-phosphate, and CO2 are generated. This pathway is a substrate for glutathione reductase, fatty acid synthesis, cytochrome P450 monooxygenases, and deoxynucleotide synthesis. |
ppp nonoxidative pathway | The second pathway is the nonoxidative pathway. This pathway synthesizes ribose 5-phosphate. |
ppp s/p/c/r | Substrates: Glucose-6-phosphate and NADP+ Products: NADPH, CO2, ribose-5-phosphate, fructose-6-phosphate, and glyceraldehyde-3-phosphate Control Enzymes: Glucose-6-P dehydrogenase Regulation: Level of NADPH (product inhibition) (feedback inhibition) |
ppp feedback inhibition | Feedback inhibition: cells keep a high level of NADPH to push reactions forward, when levels of NADPH are high, oxidative pathway is inhibited. When NADPH levels are low, oxidative pathway is activated. |
ppp compartment/tissues of interest | Compartment(s): Cytosol Tissues of interest: Every cell in the body |
gluconeogenesis f/s/p | Functions: To synthesize glucose from amino acids, lactate & glycerol in order to maintain blood glucose levels. Substrates: Amino acids, lactate, glycerol, ATP, GTP, NADH Product: Blood glucose, ADP, GDP, NAD+ |
gluconeogenesis control enzymes and regulation | Control Enzymes: Fructose 1,6 bisphosphatase Regulation: PEP carboxykinase (induced primarily by cortisol, but also by all the other counterregulatory hormones) |
gluconeogenesis compartments and tissues of interest | Compartment(s): Pyruvate to Oxaloacetate takes place inside the mitochondria, remainder in cytosol. Glucose 6-phosphatase is in the ER Tissues of interest: Liver mainly. In extreme starvation, the kidney cortex may produce glucose mostly for itself |