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Culture Media

MediaOrganism/ComponentsReaction
MacConkey Agar (MAC) Selective for intestinal pathogens, which are mostly GN RODS.(Enterobacteriaceae) Differentiate those that can FERMENT LACTOSE from those who cannot. Inhibits GP organisms. Lactose fermenters=purple/pink/red colonies Non-lactose fermenters=light pink/colorless E. coli= pink colonies with a surrounding darker pink area of precipitated bile salts.
MacConkey with Sorbitol (SMAC) Differentiate nonpathogenic E. coli that FERMENT SORBITOL WELL from the O57:H7 strain which does not. Sorbitol fermenters=purple/pink/red colonies Non-sorbitol fermenters (O57:H7)=light pink/colorless
Hektoen Enteric Agar (HEK, HE) Enhance the growth of Salmonella and Shigella by suppressing the growth of GP organisms and GN normal flora. Salmonella and Shigella (Non-lactose fermenters)= remain blue-green color of media. Lactose fermenters producing acid= orange-salmon/pink colored H2S producing Salmonella= black precipitate
Campy Agar Enhances recovery of Campylobacter by reducing oxygen. Antibiotics prevent other GN rods and yeast from growing Selective for Campylobacter
XLD Agar Isolation and differentiation of Salmonella and Shigella from other GNRs. Lactose Fermenters (nonpathogens)= yellow Salmonella=H2S=Black colonies Shigella= colorless-pink/red
Chocolate Agar (CHOC) Heated BA, Red blood cell lysis. Cultivation of fastidious organisms that require heme (Factor X) and NAD (Factor V). Pathogenic Neisseria and Haemophilus (Don't grow on BA) Haemophilus=mucoid, convex and smooth grey or transparent colonies.
Columbia Agar (CNA) Selective isolation of GPCs. Colistin (C) and Nalidixic Acid (NA) suppress growth of most GN organisms. Differentiation of colonies based on hemolysis
Bile Esculin Agar (BEA) Differential isolation and presumptive ID of Group D Streptococci and Enterococci. Sodium desoxycholate inhibits many bacteria. Growth in the presence 40% bile and hydrolysis of esculin= BROWN color change
Bordet-Gengou Agar Potato-glycerol-based medium enriched with 15-20% defibrinated blood. Isolation of Bordetella pertussis Bordetella pertussis
Buffered Charcoal Yeast Extract Agar (BCYE) Enrichment for Legionella spp. Legionella
Cefsulodin-irgasan-novobiocin Agar (CIN) Selective for Yersinia spp. May be useful for isolation of Aeromonas spp. Yersinia enterocolitica=Bull's eye colonies
Cystine-Tellurite Blood Agar Isolation of Corynebacterium diphtheriae Reduction of potassium tellurite by C. diphtheriae produces BLACK colonies.
Eosin Methylene Blue Agar (EMB) Isolation and differentiation of lactose-fermenting and non-lactose-fermenting enteric bacilli. Selective for GN organisms, against GP. E. coli=Green sheen Lactose fermenters= Dark purple Non-lactose-fermenters= colorless to light purple. Aerobacter aerogenes= colonies for BROWN centers.
Mannitol Salt Agar (MSA) Selective isolation of Staphylococci. Salt concentration of 7.5% inhibits most bacteria. Pathogenic S. aureus=Mannitol fermentation=YELLOW Non-pathogenic Staphylococci=RED
Phenylethyl Alcohol Agar (PEA) Selective isolation of GPC and Anaerobic GNR. Phenylehtanol inhibits GN organisms, Good growth= probable Staphylococcus, Streptococcus, Enterococcus, or Lactococcus. Poor growth= Probable GN organisms.
Thiosulfate Citrate-Bile Salts Agar (TCBS) Selective and differential for Vibrios Vibrio cholerae= Yellow-Orange colonies Vibrio parahaemolyticus= Green colonies
Thayer Martin Agar (MTM) Selective for N. gonorrhoeae and N. meningitidis. N. gonorrhoeae= small, translucent grey-white, mucoid colonies. N. meningitidis= convex, glistening, elevated colonies,
Selenite Broth Enrichment of isolation of Salmonella spp.
Blood Agar (BA, SBA, BAP) Supports growth of most bacteria including Staph aureus, Listeria monocytogenes, and yeasts. Hemolysis-Alpha, Beta, Gamma Cultivation of fastidious organisms.
Created by: Jennlake
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