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chapter 41
Anaerobes
Question | Answer |
---|---|
MO that will not grow in oxygen | obligate of strict anaerobe |
anaerobes are killed in a few minutes when exposed to | oxygen |
proper collection is important with anaerobes because they are present in large number in | normal flora |
collection techniques must avoid the contamination of the specimen by | normal anerobic flora |
anaerobes are found | deep in the body (so no O2) |
how many types of anaerobic transport systems are there? | 3 |
rubber stoppered collection vials are used for | liquid specimens |
oxygen free collections tubs for | swab specimens |
self contained anaerobic bag for | tissue specimens |
some gram positive anaerobes will stain | pink |
gram staining will improve in an | anaerobic chamber |
cannot be used when processing anaerobes | candle jars |
specimens may be processed in a | biological safety cabinet after incubation |
anaerobes are incubated in | anaerobic jars or pouches |
anaeobic conditions are maintained by a flow of | N2 gas |
Anaerobic media should be pre reduced by | being stored under anaerobic conditions |
cycloserine cefoxitin fructose agar or egg yolk agar is used for | Clostridium difficile |
purchased pre reduced media | pras |
pras stands for | prereduced, anaerobically sterilized media |
name for gram neg, anaerobic rods | bacteroide |
what grows on Laked kanamycin-vancomycin blood agar? | bacteroides and Prevotella |
anaerobes grow | slowly |
they only generate | 2 atp |
controls for anerobic condtions | Clostridium, colorless in the absence of oxygen |
plates that show no growth are incubated for | 5 additional days |
Anaerobes give off identifying | gases |
gases can be analyzed for identification by | Gas liquid chromatography and High resolution gas chromatography |