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BordetellaLegionella
Microbiology Respiratory Pathogens
Question | Answer |
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What disease does Bordetella pertussis cause, what is a hallmark of the infection, and how is it treated? | Bordetella pertussis causes Whooping Cough, Lymphocytosis is a hallmark of this infection, and it is treated with Erythromycin for 2 weeks |
What are the three stages of a Bordetella pertussis infection and which stage is most contagious? | 1. Catarrhal - fever, cough, runny nose, sneezing ("Flu-like symptoms") , highly contagious 2. Paroxysomal - deep convulsive coughs with ropey mucus and cyanosis 3. Convalescent - decreased severity and frequency of coughs over 2 week period |
What agar is used for Bordetella and what does it do? What if the organism needed to be transported? | Bordet-Gengou agar is specifically used for Bordetella pertissis because it reduces the overgrowth of gram positive organisms. If the organism needs to be transported, Regan-Lowe media is used with 1% casein and stored at 4 degrees C within 24 hours. |
What disease(s) does Legionella pneumophila cause and what is the treatment? | Legionella causes Legionnaire's Disease, community-acquired pneumonia, and "Pontiac Fever". The treatment for this infection is Erythromycin. |
What are the best growth conditions and the most suitable specimens to culture for Legionella? | Legionella grows best on Buffered Charcoal Yest Extract (BCYE) agar with CO2 because it provides L-cysteine and iron salts required for growth. The best specimens to culture are lung tissue, transtracheal aspirates, and pleural fluid. |